Wu Tao T, Qu Jianan, Cheung Tak Hong, Yim So Fan, Wong Yick Fu
Opt Express. 2005 Jun 27;13(13):4963-73. doi: 10.1364/opex.13.004963.
Acetic acid, inducing transient whitening (acetowhitening) when applied to epithelial tissues, is a commonly used contrast agent for detecting early cervical cancer. The goals of this research are to investigate the temporal characteristics of acetowhitening process in cervical epithelial tissue at cellular level and develop a clear understanding of the diagnostic information carried in the acetowhitening signal. A system measuring time-resolved reflectance was built to study the rising and decay processes of acetowhitening signal from the monolayered cell cultures of normal and cancerous cervical squamous cells. It is found that the dynamic processes of acetowhitening in normal and cancerous cells are significantly different. The results of this study provide insight valuable to further understand the acetowhitening process in epithelial cells and to encourage the development of an objective procedure to detect the early cervical cancers based on quantitative monitoring of the dynamic process of acetowhitening.
乙酸应用于上皮组织时会引起短暂变白（醋酸白现象），是检测早期宫颈癌常用的造影剂。本研究的目的是在细胞水平上研究宫颈上皮组织中醋酸白现象的时间特征，并深入了解醋酸白信号所携带的诊断信息。构建了一个测量时间分辨反射率的系统，以研究来自正常和癌性宫颈鳞状细胞单层细胞培养物的醋酸白信号的上升和衰减过程。发现正常细胞和癌细胞中醋酸白现象的动态过程存在显著差异。本研究结果为进一步了解上皮细胞中的醋酸白现象以及基于对醋酸白动态过程的定量监测开发检测早期宫颈癌的客观程序提供了有价值的见解。
