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基于光子晶体光纤光源的色氨酸短波长双光子激发荧光显微镜术

Short-wavelength two-photon excitation fluorescence microscopy of tryptophan with a photonic crystal fiber based light source.

作者信息

Palero J, Boer V, Vijverberg J, Gerritsen H, Sterenborg H J C M

出版信息

Opt Express. 2005 Jul 11;13(14):5363-8. doi: 10.1364/opex.13.005363.

DOI:10.1364/opex.13.005363
PMID:19498530
Abstract

We report on a novel and simple light source for short-wavelength two-photon excitation fluorescence microscopy based on the visible nonsolitonic radiation from a photonic crystal fiber. We demonstrate tunability of the light source by varying the wavelength and intensity of the Ti:Sapphire excitation light source. The visible nonsolitonic radiation is used as an excitation light source for two-photon fluorescence microscopy of tryptophan powder.

摘要

我们报道了一种基于光子晶体光纤的可见非孤子辐射的新型简单光源，用于短波长双光子激发荧光显微镜。我们通过改变钛宝石激发光源的波长和强度来证明该光源的可调谐性。可见非孤子辐射被用作色氨酸粉末双光子荧光显微镜的激发光源。

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