Department of Food Biosciences, School of Chemistry, Food Biosciences & Pharmacy, The University of Reading, Whiteknights PO Box 226, Reading RG6 6AP, United Kingdom.
Clin Nutr. 2009 Oct;28(5):556-64. doi: 10.1016/j.clnu.2009.05.008. Epub 2009 Jun 5.
BACKGROUND & AIMS: This study investigated the influence of four commercial lipid emulsions, Ivelip, ClinOleic, Omegaven and SMOFlipid((R)), on lipid body formation, fatty acid composition and eicosanoid production by cultured human peripheral blood polymorphonuclear cells (PMN) and mononuclear cells (PBMC).
PMN and PBMC were exposed to emulsions at concentrations ranging from 0.01 to 0.04%. Lipid body formation was assessed by microscopy, fatty acid composition by gas chromatography and eicosanoids by ELISA.
Stimulation of inflammatory cells and exposure to lipid emulsions promoted the formation of lipid bodies, but there did not appear to be differential effects of the emulsions tested. In contrast, there were differential effects of lipid emulsions on eicosanoid formation, particularly with regards to LTB(4) production by PMN. Omegaven dramatically increased production of eicosanoids compared with the other emulsions in a dose-dependent manner. This effect was associated with a significantly higher level of lipid peroxides in the supernatants of cells exposed to Omegaven.
Stimulation of inflammatory cells and exposure to lipid emulsions promotes lipid body formation and eicosanoid production, although the differential effects of different emulsions appear to be largely due to lipid peroxidation of unsaturated fatty acids in some emulsions in this in vitro system.
本研究旨在探讨四种市售脂肪乳剂（Ivelip、ClinOleic、Omegaven 和 SMOFlipid((R)))对培养的人外周血多形核细胞（PMN）和单核细胞（PBMC）中脂滴形成、脂肪酸组成和类二十烷酸生成的影响。
PMN 和 PBMC 分别以 0.01 至 0.04%的浓度暴露于各脂肪乳剂中。通过显微镜评估脂滴形成，通过气相色谱法评估脂肪酸组成，通过 ELISA 评估类二十烷酸生成。
炎性细胞的刺激和脂肪乳剂的暴露均促进了脂滴的形成，但似乎没有测试的乳剂的差异作用。相比之下，脂肪乳剂对类二十烷酸的生成具有不同的影响，特别是对 PMN 中 LTB(4)的生成。与其他乳剂相比，Omegaven 以剂量依赖的方式显著增加了类二十烷酸的生成。这种效应与暴露于 Omegaven 的细胞上清液中脂质过氧化物水平的显著升高有关。
炎性细胞的刺激和脂肪乳剂的暴露促进了脂滴形成和类二十烷酸的生成，尽管不同乳剂的差异作用在很大程度上归因于某些乳剂中不饱和脂肪酸的脂质过氧化。
