Rubini A S, Paduan K S, Martins T F, Labruna M B, O'Dwyer L H
Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista Julio de Mesquita Filho, Distrito de Rubião Jr s/n, CEP 18618-000, Botucatu, São Paulo, Brazil.
Vet Parasitol. 2009 Oct 14;164(2-4):324-7. doi: 10.1016/j.vetpar.2009.05.009. Epub 2009 May 19.
The present study aimed to evaluate under controlled conditions the acquisition of Hepatozoon canis by Amblyomma ovale after feeding on infected dogs, and the subsequent induction of infection in uninfected dogs that ingested the experimentally infected ticks. Two H. canis naturally infected dogs were infested with A. ovale adult ticks derived from an uninfected laboratory tick colony. After feeding, two A. ovale females presented H. canis oocysts in the hemolymph at the first and fourth days after removal of ticks from dogs. The oocysts had an average size of 244.34 microm x 255.46 microm. Three uninfected dogs were fed with ticks previously fed on the infected dogs. Only one dog became infected 32 days after oral inoculation, presenting circulating gametocytes, parasitemia less than 1%, and positive PCR confirmed to be H. canis by DNA sequencing. The results obtained indicated A. ovale ticks as potential vector of H. canis in rural areas of Brazil.
本研究旨在评估在受控条件下，卵形钝缘蜱吸食感染犬后对犬肝簇虫的获取情况，以及随后将实验感染蜱喂食给未感染犬后诱导感染的情况。两只自然感染犬肝簇虫的犬被卵形钝缘蜱成虫寄生，这些成虫来自未感染的实验室蜱虫群体。喂食后，从犬身上移除蜱虫后的第一天和第四天，两只卵形钝缘蜱雌性个体的血淋巴中出现了犬肝簇虫卵囊。卵囊的平均大小为244.34微米×255.46微米。三只未感染的犬被喂食此前已吸食感染犬血液的蜱虫。仅一只犬在口服接种32天后被感染，出现循环配子体，寄生虫血症低于1%，经DNA测序，PCR检测呈阳性，确诊为犬肝簇虫。所得结果表明，卵形钝缘蜱是巴西农村地区犬肝簇虫的潜在传播媒介。
