Silva Abraão Isaque da, Franco Eliz Oliveira, Calchi Ana Cláudia, Santos Francisco Chagas Bezerra Dos, Verde Rair de Sousa, de Mello Victória Valente Califre, Lee Daniel Antônio Braga, Secato Caroline Tostes, Dias Clara Morato, Machado Rosangela Zacarias, Roque André Luiz Rodrigues, André Marcos Rogério
Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, São Paulo State University, Jaboticabal CEP 14884-900, Brazil.
Laboratory of Trypanosomatid Biology, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21040-900, Brazil.
Pathogens. 2025 May 25;14(6):527. doi: 10.3390/pathogens14060527.
Bats are considered reservoirs of several emerging zoonotic pathogens. Previous studies on blood parasites such as Anaplasmataceae, hemoplasmas, piroplasmids, and spp. in South American bats have revealed high genetic diversity. This study investigated the molecular occurrence of these agents in 278 bats of 32 species captured in the state of Acre in the Western Brazilian Amazon. Spleen DNA samples were screened by PCR for different pathogens and tested negative for spp., spp., spp., piroplasmids, and spp. However, 84 of the 208 samples (40.4%) were positive for hemoplasmas based on the 16S rRNA gene, and 15 out of the 84 (17.85%) were positive for the 23S rRNA gene. Seventeen 16S rRNA sequences, corresponding to 12 genotypes, were grouped with hemotropic sp. previously detected in bats from Brazil, Peru, and Belize. Three 23S rRNA sequences represent three distinct genotypes clustered with hemotropic sp., previously detected in . This is the first molecular report of hemoplasmas in six bat species, namely , , , , and .
蝙蝠被认为是几种新出现的人畜共患病原体的宿主。此前对南美蝙蝠血液寄生虫（如无形体科、血支原体、梨形虫和其他物种）的研究显示出高度的遗传多样性。本研究调查了在巴西亚马逊西部阿克里州捕获的32种278只蝙蝠中这些病原体的分子存在情况。通过PCR对脾脏DNA样本进行不同病原体的筛查，结果显示对其他物种、梨形虫和其他物种检测为阴性。然而，基于16S rRNA基因，208个样本中有84个（40.4%）血支原体呈阳性，84个样本中有15个（17.85%）23S rRNA基因呈阳性。17个16S rRNA序列，对应12种基因型，与先前在巴西、秘鲁和伯利兹蝙蝠中检测到的嗜血性支原体属归为一组。3个23S rRNA序列代表3种不同基因型，与先前在某地区检测到的嗜血性支原体属聚集在一起。这是六种蝙蝠物种（即[具体物种名称1]、[具体物种名称2]、[具体物种名称3]、[具体物种名称4]、[具体物种名称5]和[具体物种名称6]）中血支原体的首次分子报告。
