Lin Yi-Xin, Wang Lan-Lan, Yan Lu-Nan, Cai Pei, Li Bo, Wen Tian-Fu, Zeng Yong
Center of Liver Transplantation and Department of Surgery, West China Hospital, Sichuan University, Chengdu, China.
Hepatobiliary Pancreat Dis Int. 2009 Jun;8(3):241-6.
Human CD8+CD28- T-suppressor (Ts) cells have been considered to indicate a reduced need for immunosuppression in pediatric liver-intestine transplant recipients and recipients of deceased heart-kidney transplants. However, in adult-to-adult living donor liver transplantation (A-A LDLT) little information is available and the clinical significance is still unknown.
Flow cytometry was used to detect the population of CD8+CD28- Ts cells present in peripheral blood in A-A LDLT recipients (n=31), patients with end-stage liver disease (n=24) and healthy controls (n=19). Meanwhile, we tested the graft function and trough levels of immunosuppression in recipients. The clinical and follow-up data of 31 transplant recipients were analyzed.
Compared with diseased controls (P=0.007) and healthy individuals (P=0.000), a notable expansion of CD8+CD28- Ts cells was found in recipients of A-A LDLT. This was associated with graft function, levels of immunosuppression and rejection episodes.
To monitor the CD8+CD28- Ts cells levels is important to evaluate the immune state of recipients. Meanwhile, it is also important to promote expansion of CD8+CD28- Ts cells in recipients of A-A LDLT, not only to sustain good graft function and decrease the dosage of immunosuppressants, but also to reduce the occurrence of rejection.
人类CD8+CD28- T抑制细胞（Ts细胞）被认为表明小儿肝肠移植受者及已故供心-肾移植受者对免疫抑制的需求降低。然而，在成人对成人活体肝移植（A-A LDLT）中，相关信息较少，其临床意义仍不清楚。
采用流式细胞术检测A-A LDLT受者（n=31）、终末期肝病患者（n=24）和健康对照者（n=19）外周血中CD8+CD28- Ts细胞的数量。同时，我们检测了受者的移植物功能和免疫抑制的谷值水平。对31例移植受者的临床和随访数据进行了分析。
与患病对照者（P=0.007）和健康个体（P=0.000）相比，A-A LDLT受者中CD8+CD28- Ts细胞显著扩增。这与移植物功能、免疫抑制水平和排斥反应发作有关。
监测CD8+CD28- Ts细胞水平对于评估受者的免疫状态很重要。同时，促进A-A LDLT受者中CD8+CD28- Ts细胞的扩增也很重要，这不仅可以维持良好的移植物功能并减少免疫抑制剂的用量，还可以减少排斥反应的发生。
