Lindner A
Institut für Anatomie, Physiologie und Hygiene der Haustiere, Universität Bonn.
Zentralbl Veterinarmed A. 1991 Jul;38(6):433-40.
The effect of temperature and duration of storage on clinico-chemical variables was investigated in Na heparinized plasma and plasma or serum of Na heparinized whole blood and whole blood of horses, respectively. The values of AST, Gamma-GT, AP, bilirubin, cholesterol, urea, total protein, albumin, Ca, Mg and Na varied in all the sample substrates investigated at 20-22 degrees C and 4 degrees C less than +/- 10% during the observation period of up to four days. In the Na heparinized plasma samples kept at 20-22 degrees C and 4 degrees C also LDH, CK, inorganic P and K did not differ significantly from its initial values during the four days. However, in the plasma of sampled stored at 20-22 degrees C as Na heparinized whole blood, the increases of LDH, CK, P and K until the fourth day of storage reached 56%, 49% and 69% and in the serum of samples kept as whole blood 90%, 165%, 147% and 48% respectively. While at 4 degrees C in the plasma of samples stored as Na heparinized whole blood until the fourth storage day only K increased significantly (by 28%), the LDH, CK and K values in the serum of samples stored as whole blood rose to 118%, 136% and 141% respectively. In the Na heparinized plasma samples kept at -18 degrees C all the measured variables did not differ significantly from the initial values during the storage time of 10 weeks.
分别在马的肝素钠抗凝血浆、肝素钠抗凝血全血的血浆或血清以及全血中，研究了温度和储存时间对临床化学变量的影响。在长达四天的观察期内，在20 - 22摄氏度和4摄氏度下，所有研究的样本底物中谷草转氨酶（AST）、γ-谷氨酰转移酶（Gamma-GT）、碱性磷酸酶（AP）、胆红素、胆固醇、尿素、总蛋白、白蛋白、钙（Ca）、镁（Mg）和钠（Na）的值变化小于±10%。在20 - 22摄氏度和4摄氏度保存的肝素钠抗凝血浆样本中，乳酸脱氢酶（LDH）、肌酸激酶（CK）、无机磷（P）和钾（K）在四天内也与初始值无显著差异。然而，在20 - 22摄氏度下作为肝素钠抗凝血全血储存的样本血浆中，直到储存第四天，LDH、CK、P和K的增加分别达到56%、49%和69%；而作为全血保存的样本血清中，这些指标分别达到90%、165%、147%和48%。在4摄氏度下，作为肝素钠抗凝血全血储存的样本血浆中，直到储存第四天只有K显著增加（28%）；而作为全血储存的样本血清中，LDH、CK和K的值分别升至118%、136%和141%。在-18摄氏度保存的肝素钠抗凝血浆样本中，在10周的储存期内，所有测量变量与初始值均无显著差异。
