Ogilvie G K, Engelking L R, Anwer M S
Am J Vet Res. 1985 Dec;46(12):2619-22.
Ten horses, a pony, and 13 cats were used to evaluate base-line blood ammonia, bilirubin, and urea nitrogen concentrations and to determine The effects of prolonged cold storage (-20 degrees C) before assay. Base-line plasma ammonia concentrations in cats (0.992 +/- 0.083 [SE] micrograms/ml) did not change significantly after 48 hours of storage (0.871 +/- 0.073 micrograms/ml); however, they were increased 4.2- and 13-fold after 168 and 216 hours of storage, respectively. In contrast to base-line plasma-ammonia values in cats, those of horses were significantly (0.265 +/- 0.044 micrograms/ml) lower, and significantly increased from base-line values after 48 hours of storage (0.861 +/- 0.094 micrograms/ml) and continued to increase 25.6-fold at 168 hours and 18.4-fold at 216 hours. Plasma urea nitrogen concentrations in cats (25.8 +/- 1.06 mg/dl) and horses (11.2 +/- 0.749 mg/dl) did not change significantly during 168 hours of storage. Total plasma bilirubin values from both cats (0.19 +/- 0.049 mg/dl) and horses (0.75 +/- 0.064 mg/dl) also did not change significantly during storage. These results indicate that feline plasma samples for ammonia determinations may be stored at -20 degrees C for up to 48 hours, whereas equine plasma ammonia values tend to increase during that time. The reason for the increase remains unexplained. Both feline and equine plasma urea nitrogen and total bilirubin are stable for at least 168 hours of storage at -20 degrees C.
选用10匹马、1匹小马和13只猫来评估基线血氨、胆红素和尿素氮浓度，并确定检测前长时间冷藏（-20摄氏度）的影响。猫的基线血浆氨浓度（0.992±0.083[标准误]微克/毫升）在储存48小时后（0.871±0.073微克/毫升）无显著变化；然而，在储存168小时和216小时后，分别增加了4.2倍和13倍。与猫的基线血浆氨值相比，马的基线血浆氨值显著更低（0.265±0.044微克/毫升），在储存48小时后显著高于基线值（0.861±0.094微克/毫升），并在168小时时继续增加25.6倍，在216小时时增加18.4倍。猫（25.8±1.06毫克/分升）和马（11.2±0.749毫克/分升）的血浆尿素氮浓度在168小时的储存期间无显著变化。猫（0.19±0.049毫克/分升）和马（0.75±0.064毫克/分升）的血浆总胆红素值在储存期间也无显著变化。这些结果表明，用于氨测定的猫血浆样本可在-20摄氏度下储存长达48小时，而在此期间马的血浆氨值往往会升高。升高的原因尚不清楚。猫和马的血浆尿素氮和总胆红素在-20摄氏度下至少可稳定储存168小时。
