Sivolodskiĭ E P
Zh Mikrobiol Epidemiol Immunobiol. 1991 Jul(7):14-7.
For the first time the method of the rapid (1 hour) screening of groups of gram-negative bacteria by the OF test with glucose, carried out with the use of microvolumetric techniques, has been developed. The method is based on the use of hydrogen peroxide at non-bactericidal concentration as a component of a liquid buffer medium containing indicator and glucose and intended for the oxidation of glucose. Catalase of bacteria introduced into the medium for study ensures the rapid saturation of the medium with oxygen and the completion of the oxidation of glucose in 10-60 minutes. An equal period is necessary to achieve the complete fermentation of glucose in the same medium without hydrogen peroxide. The method has been proved to yield significant results in the joint study of the OF test on 502 strains, belonging to 21 genera of fermenting and nonfermenting gram-negative bacteria, by the hydrogen peroxide method and in Hugh-Leifson medium.
首次开发了一种利用微体积技术，通过葡萄糖氧化发酵试验对革兰氏阴性菌进行快速（1小时）筛选的方法。该方法基于使用非杀菌浓度的过氧化氢作为含有指示剂和葡萄糖的液体缓冲介质的成分，用于葡萄糖的氧化。引入用于研究的培养基中的细菌过氧化氢酶可确保培养基在10 - 60分钟内迅速被氧气饱和并完成葡萄糖的氧化。在没有过氧化氢的相同培养基中，葡萄糖完全发酵也需要相同的时间。通过过氧化氢法和休-利夫森培养基对属于21个发酵和非发酵革兰氏阴性菌属的502株菌株进行氧化发酵试验联合研究，已证明该方法能产生显著结果。
