Kalina G P, Vinogradova L A, Trukhina G M
Zh Mikrobiol Epidemiol Immunobiol. 1985 Feb(2):3-11.
The scheme for the identification of Gram-negative nonfermenting microorganisms is proposed. The scheme comprises the most important key signs, such as the cytochrome oxidase reaction determined by the method of Gaby and Hadley, the oxidation/fermentation test, maltose oxidation and motility, as well as additional key signs, among them gelatinase activity, the oxidation of 10% lactose, nitratase activity with the liberation of free nitrogen, the utilization of the sources of carbon and energy (glucose and sodium acetate) in limited media containing ammonium salts and nitrates as the sources of nitrogen. Additional tests for the identification of nonfermenting microorganisms similar in their main key signs are also recommended.
提出了革兰氏阴性非发酵微生物的鉴定方案。该方案包括最重要的关键特征,如采用加比和哈德利方法测定的细胞色素氧化酶反应、氧化/发酵试验、麦芽糖氧化和运动性,以及其他关键特征,其中包括明胶酶活性、10%乳糖的氧化、游离氮释放的硝酸还原酶活性、在以铵盐和硝酸盐作为氮源的有限培养基中对碳源和能源(葡萄糖和醋酸钠)的利用。还推荐了用于鉴定主要关键特征相似的非发酵微生物的其他试验。