利用宽带相干反斯托克斯拉曼光谱法检测水中的枯草芽孢杆菌孢子。

Detection of Bacillus subtilis spores in water by means of broadband coherent anti-Stokes Raman spectroscopy.

作者信息

Petrov Georgi, Yakovlev Vladislav, Sokolov Alexei, Scully Marlan

出版信息

Opt Express. 2005 Nov 14;13(23):9537-42. doi: 10.1364/opex.13.009537.

DOI:10.1364/opex.13.009537

PMID:19503156

Abstract

Broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy is used for detection of bacterial spores in aqueous solution. Polarization CARS spectroscopy is employed to suppress the non-resonant background. CARS spectrum recorded in the spectral region from 700 to 1900 cm(-1) exhibits all the characteristic features of spontaneous Raman spectrum taken for a solid powder and resembles that one of the dipicolinic acid, which is considered to be the major component of bacterial spores, including anthrax.

摘要

宽带相干反斯托克斯拉曼散射（CARS）光谱用于检测水溶液中的细菌孢子。采用偏振CARS光谱来抑制非共振背景。在700至1900 cm(-1)光谱区域记录的CARS光谱展现出了固体粉末自发拉曼光谱的所有特征，并且类似于二吡啶甲酸的自发拉曼光谱，二吡啶甲酸被认为是包括炭疽杆菌在内的细菌孢子的主要成分。