Blumenfeld W, Gan G L
Department of Pathology, University of California-San Francisco.
Acta Cytol. 1991 Nov-Dec;35(6):710-2.
A case of histoplasmosis in a patient with acquired immunodeficiency syndrome is presented. Intracytoplasmic organisms were found in Diff-Quik- and Papanicolaou-stained bronchoalveolar lavage fluid sediment. Budding yeasts were found in the silver methenamine-stained material. It was initially thought that the yeasts might represent another infecting organism, especially in view of the patient's history of esophageal candidiasis. When the silver-stained material was counterstained with hematoxylin and eosin, simultaneous demonstration of cytologic detail and silver positivity showed that the silver-positive yeasts were intracellular, confirming that they were Histoplasma capsulatum. Counterstaining with hematoxylin and eosin allows localization of argyrophilic organisms to either intracellular or extracellular sites and is technically simple to perform. It is useful in acquired immunodeficiency syndrome patients and other immunocompromised hosts, in whom multiple organisms are often found.
本文报告1例获得性免疫缺陷综合征患者的组织胞浆菌病。在Diff-Quik染色和巴氏染色的支气管肺泡灌洗液体沉渣中发现胞浆内微生物。在亚甲胺银染色材料中发现出芽酵母。最初认为这些酵母可能代表另一种感染性生物体，尤其是考虑到患者有食管念珠菌病病史。当用苏木精和伊红对银染色材料进行复染时，同时显示出细胞学细节和银阳性，表明银阳性酵母位于细胞内，证实它们是荚膜组织胞浆菌。用苏木精和伊红进行复染可将嗜银性生物体定位在细胞内或细胞外部位，且操作技术简单。它对获得性免疫缺陷综合征患者和其他免疫功能低下宿主有用，在这些患者中常可发现多种生物体。
