Huppert M, Oliver D J, Sun S H
J Clin Microbiol. 1978 Nov;8(5):598-603. doi: 10.1128/jcm.8.5.598-603.1978.
Initial examination of hematoxylin & eosin-stained tissue from a human brain specimen did not reveal the fungi which were seen in subsequent tissue sections stained with methenamine-silver nitrate. Microabscesses seen in the hematoxylin & eosin-stained sections were not apparent in the methenamine--silver nitrate-stained tissue. Staining with methenamine--silver nitrate and counterstaining with hematoxylin & eosin proved excellent not only for detecting fungus cells, but also for revealing their relationship to the host cellular response in this case and in examples of experimental murine coccidioidomycosis and histoplasmosis.
对一份人类脑标本苏木精和伊红染色的组织进行的初步检查未发现真菌，而在随后用硝酸银六胺染色的组织切片中可见真菌。在苏木精和伊红染色切片中看到的微脓肿在硝酸银六胺染色的组织中并不明显。硝酸银六胺染色并用苏木精和伊红复染不仅能出色地检测真菌细胞，还能揭示在该病例以及实验性小鼠球孢子菌病和组织胞浆菌病实例中真菌细胞与宿主细胞反应的关系。
