Oh W Y, Bouma B E, Iftimia N, Yun S H, Yelin R, Tearney G J
Opt Express. 2006 Jan 23;14(2):726-35. doi: 10.1364/opex.14.000726.
Full-field optical coherence microscopy (FFOCM) is an interferometric technique for obtaining wide-field microscopic images deep within scattering biological samples. FFOCM has primarily been implemented in the 0.8 mum wavelength range with silicon-based cameras, which may limit penetration when imaging human tissue. In this paper, we demonstrate FFOCM at the wavelength range of 0.9 - 1.4 mum, where optical penetration into tissue is presumably greater owing to decreased scattering. Our FFOCM system, comprising a broadband spatially incoherent light source, a Linnik interferometer, and an InGaAs area scan camera, provided a detection sensitivity of 86 dB for a 2 sec imaging time and an axial resolution of 1.9 mum in water. Images of phantoms, tissue samples, and Xenopus Laevis embryos were obtained using InGaAs and silicon camera FFOCM systems, demonstrating enhanced imaging penetration at longer wavelengths.
全场光学相干显微镜（FFOCM）是一种干涉技术，用于获取散射生物样本内部深处的宽视野微观图像。FFOCM主要是在0.8微米波长范围内使用基于硅的相机实现的，这在对人体组织成像时可能会限制穿透深度。在本文中，我们展示了在0.9 - 1.4微米波长范围内的FFOCM，由于散射减少，该波长范围内光对组织的穿透可能更大。我们的FFOCM系统包括一个宽带空间非相干光源、一个林尼克干涉仪和一个铟镓砷面扫描相机，在2秒成像时间内提供了86分贝的检测灵敏度，在水中的轴向分辨率为1.9微米。使用铟镓砷和硅相机FFOCM系统获得了体模、组织样本和非洲爪蟾胚胎的图像，证明了在较长波长下成像穿透能力增强。
