Deng Q Y, Yang B, Wang J F, Whiteley C G, Wang X N
School of Biosciences and Bioengineering, South China University of Technology, Guangzhou, 510641, People's Republic of China.
Biotechnol Lett. 2009 Oct;31(10):1505-9. doi: 10.1007/s10529-009-0040-3. Epub 2009 Jun 6.
A novel biological method for the synthesis of platinum nanoparticles using the horse spleen apoferritin (HSAF) is reported. When HSAF was incubated with K(2)PtCl(6) at 23 degrees C) for 48 h followed by subsequent reduction with NaBH(4) it resulted in the formation of spherical platinum nanoparticles, size 4.7 +/- 0.9 nm, with narrow particle size distribution confirmed by transmission electron microscopy and energy dispersive X-ray analysis. As the initial platinum concentration increased through 0.155, 0.31, 0.465 to 0.62 mM the efficiency of its removal from solution by the apoferritin was 99, 99, 84 and 71% respectively. The maximum uptake of platinum salt per mM apoferritin was estimated at 12.7 mmol l(-1) h(-1). These results clearly indicate that the HSAF protein cage can successfully serve as a suitable size-constrained support matrix for the biological synthesis of platinum nanoparticles.
报道了一种使用马脾脱铁铁蛋白（HSAF）合成铂纳米颗粒的新型生物学方法。当HSAF与K₂PtCl₆在23℃下孵育48小时，随后用NaBH₄还原时，会形成球形铂纳米颗粒，尺寸为4.7±0.9纳米，通过透射电子显微镜和能量色散X射线分析证实其粒径分布狭窄。随着初始铂浓度从0.155、0.31、0.465增加到0.62 mM，脱铁铁蛋白从溶液中去除铂的效率分别为99%、99%、84%和71%。每毫摩尔脱铁铁蛋白对铂盐的最大摄取量估计为12.7 mmol l⁻¹ h⁻¹。这些结果清楚地表明，HSAF蛋白笼可以成功地作为一种合适的尺寸受限的支撑基质用于铂纳米颗粒的生物合成。
