You Xiaoyan, Liu Yiping, Jiang Xiaosong, Du Huarui, Liu Zuohua, Zhu Qing
College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan, China.
Biochem Genet. 2009 Aug;47(7-8):511-20. doi: 10.1007/s10528-009-9249-z. Epub 2009 Jun 7.
Using PCR-SSCP with five primer pairs, we detected six single nucleotide polymorphisms of the H-FABP gene: 332G --> A, 534G --> A, 783C --> T, 835C --> T, 1198T --> C, and 2329C --> T. Chi-square results showed significant differences (P < 0.05) in genotype frequency among breeds in Fragment 1 and extremely significant differences (P < 0.01) in Fragments 2-4. We found a significant association between Fragment 2 genotype and muscle fiber number, Arg and Thr (P < 0.05); between Fragment 3 genotype and living weight, carcass weight, breast muscle weight, abdominal fat weight, and abdominal fat percentage (P < 0.05); between Fragment 4 genotype and Thr, Phe, and inosinic acid (P < 0.05). It was concluded that H-FABP was the major gene influencing slaughter performance and meat quality or was linked with the major gene in these strains and that the C783T mutation could be used as a candidate molecular genetic marker for breeding selection. The combination M1C2-B2B2-D1D1 is an ideal model for breeding in these strains because it can improve slaughter and meat quality traits.
使用五对引物进行PCR-SSCP，我们检测到H-FABP基因的六个单核苷酸多态性：332G→A、534G→A、783C→T、835C→T、1198T→C和2329C→T。卡方检验结果显示，片段1中各品种间基因型频率存在显著差异（P<0.05），片段2-4中存在极显著差异（P<0.01）。我们发现，片段2基因型与肌纤维数量、精氨酸和苏氨酸之间存在显著关联（P<0.05）；片段3基因型与活体重、胴体重、胸肌重、腹脂重和腹脂率之间存在显著关联（P<0.05）；片段4基因型与苏氨酸、苯丙氨酸和肌苷酸之间存在显著关联（P<0.05）。得出的结论是，H-FABP是影响屠宰性能和肉质的主基因，或与这些品系中的主基因连锁，并且C783T突变可作为育种选择的候选分子遗传标记。组合M1C2-B2B2-D1D1是这些品系育种的理想模型，因为它可以改善屠宰和肉质性状。
