Laboratory Animal Center, Jilin University, China.
Gene. 2011 Nov 10;487(2):170-3. doi: 10.1016/j.gene.2011.07.005. Epub 2011 Aug 6.
This study was designed to investigate the single nucleotide polymorphism (SNP) in intron 1 of the gene A-FABP in 127 Junmu No. 1 white swine using PCR-SSCP. The association between the polymorphism and meat quality traits was also studied. The cloning and sequencing results indicated that the polymorphism of intron 1 was due to a point mutation in position 3481bp of A-FABP, giving 3 genotypes (CC, CD and DD). Association analysis indicated that the polymorphism had a significant effect on marbling (P<0.05). Genotype DD had higher marbling than CD and CC, but the difference between CD and CC was no significant. Polymorphism had a highly significant effect on intramuscular fat (IMF) content (P<0.01). DD was higher than CD, which was higher than CC. No significant conclusions can be drawn regarding other traits. Immunoblot analysis of A-FABP levels was carried out on 3 different genotype individuals. Expression was markedly reduced in DD compared with genotype CC. Thus A-FABP may be a candidate gene or a quantitative trait locus-linked gene associated with meat quality traits.
本研究采用 PCR-SSCP 技术检测了 127 头军牧一号白猪 A-FABP 基因内含子 1 的单核苷酸多态性（SNP），并研究了其与肉质性状的相关性。克隆测序结果表明，内含子 1 的多态性是由于在 3481bp 位置发生了点突变，导致出现 3 种基因型（CC、CD 和 DD）。关联分析表明，该多态性对大理石花纹有显著影响（P<0.05）。基因型 DD 的大理石花纹显著高于 CD 和 CC，而 CD 和 CC 之间没有显著差异。该多态性对肌内脂肪（IMF）含量有高度显著影响（P<0.01）。DD 显著高于 CD，CD 显著高于 CC。其他性状没有得出显著结论。对 3 种不同基因型个体的 A-FABP 水平进行免疫印迹分析，结果表明 DD 型的表达明显低于 CC 型。因此，A-FABP 可能是与肉质性状相关的候选基因或数量性状位点连锁基因。
