The Leicester School of Pharmacy, Faculty of Health and Life Sciences, De Montfort University, The Gateway, Leicester, LE1 9BH, UK.
Pharm Dev Technol. 2010 Jan-Feb;15(1):80-8. doi: 10.3109/10837450903002181.
Aqueous concentrated plain mixtures of dextran and concanavalin A (con A) were examined for their rheological response to glucose for comparison with previously studied partially photopolymerized acrylic derivatives. Non-destructive oscillatory tests were undertaken within the linear viscoelastic range to examine the relationship between the rheometry and the stoichiometry of the interactive materials and to examine rheological parameters as affected by molecular weight, component ratio, temperature and glucose concentrations between 0 and 1% w/w. These simple formulations were studied at 1 and 10 Hz at 0.5% strain at both 20 and 37 degrees C. A second simplified rheological test was undertaken to demonstrate gel-sol reversibility and to produce a measure of equilibria created between these gels and glucose solutions with which they are in contact. This mimics the conditions in which the gel acts as a responsive gateway in the insulin delivery device. It proved that the gels equilibrate with glucose solutions, rather than indiscriminately removing glucose. This is important in terms of producing a delivery device that can respond in a reversible, glucose concentration-dependent manner. The method used for this is capable of relative values only but provides information not obtainable from conventional rheometry.
将葡聚糖和伴刀豆球蛋白 A(con A)的水相浓缩混合物与先前研究过的部分光聚合丙烯酸衍生物的流变响应进行了比较。在线性粘弹性范围内进行了非破坏性振荡测试，以研究流变学与相互作用材料的化学计量之间的关系，并研究分子量、组成比、温度和葡萄糖浓度在 0 到 1%（w/w）之间对流变学参数的影响。这些简单的配方在 20 和 37°C 下以 1 和 10 Hz、0.5%应变进行了研究。进行了第二次简化流变测试，以证明凝胶-溶胶的可逆性，并测量与接触的葡萄糖溶液之间形成的平衡。这模拟了凝胶在胰岛素输送装置中作为响应性门户的条件。结果证明凝胶与葡萄糖溶液平衡，而不是不分青红皂白地去除葡萄糖。这对于生产能够以可逆、葡萄糖浓度依赖的方式响应的输送装置非常重要。用于此目的的方法只能提供相对值，但提供了常规流变学无法获得的信息。
