Huang K X, Sun B C, Gonon F G, Jin G Z
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Zhongguo Yao Li Xue Bao. 1991 Jan;12(1):32-6.
The extracellular (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) in rat striatum were measured by in vivo voltammetry to elucidate the effects of 3 tetrahydroprotoberberines on DA neurotransmission. l-tetrahydropalmatine (l-THP, 2 mg.kg-1) or l-stepholidine (l-SPD, 0.5 mg.kg-1) iv increased the striatal DA release by 225% and 233%, respectively; and l-SPD increased the DOPAC level by 70%. Moreover, the enantiomer d-THP (2 mg.kg-1) increased the DA release by 97%, and a large dose of d-THP (20 mg.kg-1) dramatically increased the extracellular DA by 1456% while it slightly elevated the DOPAC level by 123%. These results support the previous ideas that l-SPD and l-THP can block DA receptors and d-THP can deplete neuronal DA.
采用体内伏安法测定大鼠纹状体细胞外多巴胺（DA）和3,4 - 二羟基苯乙酸（DOPAC），以阐明3种四氢原小檗碱对DA神经传递的影响。静脉注射左旋四氢巴马汀（l-THP，2 mg·kg-1）或左旋千金藤啶碱（l-SPD，0.5 mg·kg-1）分别使纹状体DA释放增加225%和233%；l-SPD使DOPAC水平升高70%。此外，对映体d-THP（2 mg·kg-1）使DA释放增加97%，大剂量d-THP（20 mg·kg-1）使细胞外DA显著增加1456%，而DOPAC水平仅轻微升高123%。这些结果支持了之前的观点，即l-SPD和l-THP可阻断DA受体，而d-THP可消耗神经元内的DA。
