Boot R, Vlemminx M J, Reubsaet F A G
Section of Laboratory Animal Microbiology, BA Bilthoven The Netherlands.
Lab Anim. 2009 Oct;43(4):371-5. doi: 10.1258/la.2009.0070131. Epub 2009 Jun 8.
Monitoring of rodents for Pasteurellaceae infection may be carried out by the polymerase chain reaction (PCR). We tested which of 17 rodent Pasteurellaceae strains were detected by three PCR primer sets. By phylogenetic analysis, 12 strains were assigned to the Rodent cluster and five strains to other clusters, namely the Somnus cluster, Pasteurella sensu stricto, Actinobacillus sensu stricto, the Mannheimia and Rossii cluster. A primer set developed to detect biotype Heyl [Pasteurella] pneumotropica produced amplicons from three strains and appeared specific for this taxon. A primer set developed to detect biotype Jawetz [P.] pneumotropica produced amplicons from the [P.] pneumotropica type strain and two other strains within the Rodent cluster. A primer set as described by Bootz and his co-workers (Bootz F, Kirschnek S, Nicklas W, Wyss SK, Homberger FR. Detection of Pasteurellaceae in rodents by polymerase chain reaction analysis. Lab Anim Sci 1998;48:542-6) for the detection of all Pasteurellaceae indeed detected all bacterial strains examined. Bootz's primer set should be used to monitor rodents for Pasteurellaceae infection by PCR as FELASA recommends the monitoring of rodents for all Pasteurellaceae taxa. Health monitoring reports should specify the primer set(s) used for PCR testing rodents for Pasteurellaceae infection.
可通过聚合酶链反应（PCR）对啮齿动物进行巴氏杆菌科感染监测。我们测试了三种PCR引物组能检测出17种啮齿动物巴氏杆菌科菌株中的哪些菌株。通过系统发育分析，12个菌株被归入啮齿动物簇，5个菌株被归入其他簇，即睡眠菌簇、狭义巴斯德菌、狭义放线杆菌、曼氏杆菌和罗西氏菌簇。一种为检测嗜肺巴氏杆菌[巴斯德菌]海勒生物型而开发的引物组从三种菌株中产生了扩增子，且似乎对该分类单元具有特异性。一种为检测嗜肺巴氏杆菌[巴氏杆菌]雅韦茨生物型而开发的引物组从嗜肺巴氏杆菌模式菌株以及啮齿动物簇内的另外两种菌株中产生了扩增子。Bootz及其同事（Bootz F、Kirschnek S、Nicklas W、Wyss SK、Homberger FR。通过聚合酶链反应分析检测啮齿动物中的巴氏杆菌科。实验动物科学1998；48：542 - 6）描述的用于检测所有巴氏杆菌科的引物组确实检测出了所有检测的细菌菌株。应按照FELASA建议对所有巴氏杆菌科分类单元监测啮齿动物的要求，使用Bootz的引物组通过PCR监测啮齿动物的巴氏杆菌科感染。健康监测报告应指明用于PCR检测啮齿动物巴氏杆菌科感染的引物组。
