Tiam1和Rac1过表达在鼻咽癌中的预后价值
Prognostic value of Tiam1 and Rac1 overexpression in nasopharyngeal carcinoma.
作者信息
Qi Yan, Huang Bo, Yu Linlin, Wang Qi, Lan Guiping, Zhang Qiuhang
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, XuanWu Hospital, Capital Medical University, Beijing 100053, China.
出版信息
ORL J Otorhinolaryngol Relat Spec. 2009;71(3):163-71. doi: 10.1159/000223440. Epub 2009 Jun 9.
PURPOSE
This study is designed to investigate the roles of Tiam1 and Rac1 in nasopharyngeal carcinoma (NPC).
METHODS
NPC samples (n = 102) were analyzed with immunohistochemistry for Tiam1 and Rac1 proteins, 28 of which were also analyzed with quantitative RT-PCR and Western blots for Tiam1 and Rac1 mRNA and protein expression. The expression of Tiam1 and Rac1 in noncancerous nasopharyngeal tissue (n = 26) acted as a control. The expression was gauged regarding stage, grade and survival.
RESULTS
Tiam1 and Rac1 were overexpressed in NPC cells and their protein upregulation was significantly associated with stage and grading (p < 0.05 for all). Cox regression analysis revealed Tiam1 and Rac1 protein upregulation was correlated with lower disease-free and overall survival rates (p < 0.001).
CONCLUSIONS
Upregulation of Tiam1 and Rac1 proteins may play a critical role in tumor progression of NPC, and work as a prognostic factor for NPC patients.
目的
本研究旨在探讨Tiam1和Rac1在鼻咽癌（NPC）中的作用。
方法
采用免疫组织化学方法分析102例NPC样本中Tiam1和Rac1蛋白的表达情况，其中28例还采用定量RT-PCR和Western印迹法分析Tiam1和Rac1 mRNA及蛋白表达。以26例非癌性鼻咽组织作为对照。根据分期、分级和生存率评估其表达情况。
结果
Tiam1和Rac1在NPC细胞中过表达，其蛋白上调与分期和分级显著相关（均P < 0.05）。Cox回归分析显示，Tiam1和Rac1蛋白上调与无病生存率和总生存率降低相关（P < 0.001）。
结论
Tiam1和Rac1蛋白的上调可能在NPC的肿瘤进展中起关键作用，并可作为NPC患者的预后因素。
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