Qi Yan, Huang Bo, Yu Linlin, Wang Qi, Lan Guiping, Zhang Qiuhang
Department of Otorhinolaryngology Head and Neck Surgery, XuanWu Hospital, Capital Medical University, Beijing 100053, China.
ORL J Otorhinolaryngol Relat Spec. 2009;71(3):163-71. doi: 10.1159/000223440. Epub 2009 Jun 9.
This study is designed to investigate the roles of Tiam1 and Rac1 in nasopharyngeal carcinoma (NPC).
NPC samples (n = 102) were analyzed with immunohistochemistry for Tiam1 and Rac1 proteins, 28 of which were also analyzed with quantitative RT-PCR and Western blots for Tiam1 and Rac1 mRNA and protein expression. The expression of Tiam1 and Rac1 in noncancerous nasopharyngeal tissue (n = 26) acted as a control. The expression was gauged regarding stage, grade and survival.
Tiam1 and Rac1 were overexpressed in NPC cells and their protein upregulation was significantly associated with stage and grading (p < 0.05 for all). Cox regression analysis revealed Tiam1 and Rac1 protein upregulation was correlated with lower disease-free and overall survival rates (p < 0.001).
Upregulation of Tiam1 and Rac1 proteins may play a critical role in tumor progression of NPC, and work as a prognostic factor for NPC patients.
本研究旨在探讨Tiam1和Rac1在鼻咽癌（NPC）中的作用。
采用免疫组织化学方法分析102例NPC样本中Tiam1和Rac1蛋白的表达情况，其中28例还采用定量RT-PCR和Western印迹法分析Tiam1和Rac1 mRNA及蛋白表达。以26例非癌性鼻咽组织作为对照。根据分期、分级和生存率评估其表达情况。
Tiam1和Rac1在NPC细胞中过表达，其蛋白上调与分期和分级显著相关（均P < 0.05）。Cox回归分析显示，Tiam1和Rac1蛋白上调与无病生存率和总生存率降低相关（P < 0.001）。
Tiam1和Rac1蛋白的上调可能在NPC的肿瘤进展中起关键作用，并可作为NPC患者的预后因素。
