Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China.
Oncol Rep. 2011 Mar;25(3):841-8. doi: 10.3892/or.2010.1122. Epub 2010 Dec 24.
Accumulating evidence has demonstrated that T-cell lymphoma invasion and metastasis 1 (Tiam1) plays an important role in the occurrence and development of several different tumors; however, to date, little research has been done to verify the potential role of Tiam1 as a prognostic marker for esophageal squamous cell carcinoma (ESCC). In the present study, we examined the expression of Tiam1 in ESCC tissues by immunohistochemistry, in situ hybridization, semi-quantitative RT-PCR and Western blotting methods and investigated the correlation between Tiam1 levels and prognosis of patients with ESCC. Tiam1 exhibited high expression in ESCC tissues, whereas the normal esophageal tissues showed negative or weak Tiam1 expression. Additionally, Tiam1 mRNA and protein expression levels were both significantly correlated with histology grade, clinical staging and lymph node metastasis (all P<0.05), but not related to age and gender (both P>0.05). Further, ESCC patients with strong Tiam1 mRNA (P=0.000) and protein (P=0.000) expression had a poorer prognosis than those with weak expression. These findings demonstrate that Tiam1 may be used as molecular marker for predicting the prognosis of patients with ESCC.
越来越多的证据表明，T 细胞淋巴瘤侵袭转移因子 1（Tiam1）在几种不同肿瘤的发生和发展中发挥着重要作用；然而，迄今为止，很少有研究证实 Tiam1 作为食管鳞状细胞癌（ESCC）的预后标志物的潜在作用。在本研究中，我们通过免疫组织化学、原位杂交、半定量 RT-PCR 和 Western blot 方法检测了 Tiam1 在 ESCC 组织中的表达，并探讨了 Tiam1 水平与 ESCC 患者预后之间的相关性。Tiam1 在 ESCC 组织中高表达，而正常食管组织呈阴性或弱阳性表达。此外，Tiam1 mRNA 和蛋白表达水平均与组织学分级、临床分期和淋巴结转移显著相关（均 P<0.05），但与年龄和性别无关（均 P>0.05）。进一步研究发现，Tiam1 mRNA 表达较强（P=0.000）和蛋白表达较强（P=0.000）的 ESCC 患者预后较差。这些发现表明，Tiam1 可作为预测 ESCC 患者预后的分子标志物。
