Fraune Sebastian, Abe Yuichi, Bosch Thomas C G
Christian-Albrechts University Kiel, Zoological Institute, Olshausenstrasse 40, Kiel, Germany.
Environ Microbiol. 2009 Sep;11(9):2361-9. doi: 10.1111/j.1462-2920.2009.01963.x. Epub 2009 Jun 7.
Microbes have profound influence on the biology of host tissue. Imbalances in host-microbe interaction underlie many human diseases. Little, however, is known about how epithelial homeostasis affects associated microbial community structure. In Hydra, the epithelium actively shapes its microbial community indicating distinct selective pressures imposed on the epithelium. Here, using a mutant strain of Hydra magnipapillata we eliminated all derivatives of the interstitial stem cell lineage while leaving both epithelial cell lineages intact. By bacterial 16S rRNA gene analysis we observed that removing gland cells and neurones from the epithelium causes significant changes in hydra's microbial community. Absence of interstitial stem cells and nematocytes had no affect on the microbiota. When compared with controls, animals lacking neurones and gland cells showed reduced abundance of beta-Proteobacteria accompanied by a significantly increased abundance of a Bacteroidetes bacterium. This previously unrecognized link between cellular tissue composition and microbiota may be applicable to understanding mechanisms controlling host-microbe interaction in other epithelial systems.
微生物对宿主组织的生物学特性具有深远影响。宿主与微生物相互作用的失衡是许多人类疾病的根源。然而，关于上皮细胞稳态如何影响相关微生物群落结构，我们知之甚少。在水螅中，上皮细胞积极塑造其微生物群落，这表明上皮细胞受到了独特的选择压力。在这里，我们使用巨型多乳头水螅的突变菌株，消除了间质干细胞谱系的所有衍生物，同时保持两个上皮细胞谱系完整。通过细菌16S rRNA基因分析，我们观察到从上皮细胞中去除腺细胞和神经元会导致水螅微生物群落发生显著变化。间质干细胞和刺细胞的缺失对微生物群没有影响。与对照组相比，缺乏神经元和腺细胞的动物中β-变形菌的丰度降低，同时一种拟杆菌的丰度显著增加。这种细胞组织组成与微生物群之间以前未被认识到的联系，可能适用于理解其他上皮系统中控制宿主-微生物相互作用的机制。
