Gyongyossy-Issa M I C, Zhang J G, Culibrk B, Hunter F, Levin E, Scammell K, Weiss S, Holmes D L, Holme S
Canadian Blood Services, UBC Center for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada.
Vox Sang. 2009 Aug;97(2):102-9. doi: 10.1111/j.1423-0410.2009.01196.x. Epub 2009 May 11.
In Europe, buffy-coat processing allows for the use of platelet additive solutions (PAS). These solutions, however, have long been questioned for their lack of glucose, a potentially essential nutrient for platelet storage. Using a novel, practical, two-part system for incorporation of glucose into an additive solution (PAS-G), this study compares platelet storage in plasma to storage in PAS-G.
A paired study design of platelet concentrates (PC) were prepared from leucoreduced pools of eight buffy coats (BCP) split into two equal pools, with suspension in autologous plasma, or PAS-G. On days 2, 5, 7 and 9 of storage, samples were tested using standard in vitro platelet parameters. Data were analysed by paired Student's t-tests.
During storage, PCs in PAS-G maintain a quality profile that is strikingly similar to PCs stored in plasma in terms of platelet activation (CD62) morphology score, swirl, glucose metabolism and pH. However, PCs in PAS-G perform lower (P < 0.05) in the %ESC and %HSR assays.
PAS-G's novel presentation allows incorporation of glucose into the additive solution so that it is roughly equivalent to plasma for the maintenance of buffy-coat PCs.
在欧洲，白膜层处理允许使用血小板添加剂溶液（PAS）。然而，长期以来这些溶液因其缺乏葡萄糖（一种对血小板储存可能至关重要的营养素）而受到质疑。本研究使用一种新颖、实用的两部分系统将葡萄糖掺入添加剂溶液（PAS-G）中，比较了血小板在血浆中的储存与在PAS-G中的储存情况。
采用配对研究设计，从8个白膜层（BCP）的白细胞去除库中制备血小板浓缩物（PC），将其分成两个相等的库，分别悬浮于自体血浆或PAS-G中。在储存的第2、5、7和9天，使用标准体外血小板参数对样本进行检测。数据采用配对学生t检验进行分析。
在储存期间，PAS-G中的PC在血小板活化（CD62）形态评分、漩涡、葡萄糖代谢和pH值方面保持与血浆中储存的PC惊人相似的质量概况。然而，PAS-G中的PC在%ESC和%HSR检测中的表现较低（P<0.05）。
PAS-G的新颖配方允许将葡萄糖掺入添加剂溶液中，使其在维持白膜层PC方面大致等同于血浆。
