He Xiaoyin, Liu Jun
Department of Biomedical Engineering, Ohio State University, Columbus, Ohio, USA.
Invest Ophthalmol Vis Sci. 2009 Nov;50(11):5148-54. doi: 10.1167/iovs.09-3439. Epub 2009 Jun 10.
To describe a quantitative ultrasonic spectroscopy technique for the noninvasive characterization of corneal biomechanical properties and to compare these measurements with established techniques in a porcine eye model.
An ultrasound system was constructed to accurately acquire acoustic reflections from corneas through a saline bath. Corneal properties (including thickness, density and aggregate modulus) were estimated from the measured reflection spectra based on wave propagation analysis. Twenty fresh porcine corneas were measured using the quantitative ultrasound method and other established techniques that can only be applied to dissected corneas.
The ultrasonic measurements of corneal thickness and aggregate modulus were significantly correlated with the measurements of established techniques (Pearson's correlation = 0.99 and 0.61; P < 0.005), and good sample-to-sample consistency was achieved. The measurement of corneal density agreed well in terms of mean and range, but the correlation did not achieve statistical significance (Pearson's correlation = 0.41; P = 0.07).
The ultrasonically measured corneal biomechanical properties agreed well with the measurements obtained by using established techniques, validating the accuracy of the ultrasound method. Quantitative ultrasound spectroscopy may provide a noninvasive approach for in vivo characterization of corneal biomechanical properties.
描述一种用于角膜生物力学特性无创表征的定量超声光谱技术，并在猪眼模型中将这些测量结果与既定技术进行比较。
构建了一个超声系统，通过盐水浴准确获取来自角膜的声反射。基于波传播分析，从测量的反射光谱中估计角膜特性（包括厚度、密度和聚集模量）。使用定量超声方法和其他仅适用于解剖角膜的既定技术对20个新鲜猪角膜进行测量。
角膜厚度和聚集模量的超声测量结果与既定技术的测量结果显著相关（皮尔逊相关系数分别为0.99和0.61；P < 0.005），并实现了良好的样本间一致性。角膜密度的测量在均值和范围方面吻合良好，但相关性未达到统计学显著性（皮尔逊相关系数 = 0.41；P = 0.07）。
超声测量的角膜生物力学特性与使用既定技术获得的测量结果吻合良好，验证了超声方法的准确性。定量超声光谱法可能为体内角膜生物力学特性的表征提供一种无创方法。
