Department of Animal Science, University of California, Davis, CA 95616, USA.
Anim Genet. 2009 Dec;40(6):878-82. doi: 10.1111/j.1365-2052.2009.01934.x. Epub 2009 Jun 5.
Identification of the genes and polymorphisms underlying quantitative traits, and understanding how these genes and polymorphisms affect economic traits, are important for successful marker-assisted selection and more efficient management strategies in commercial cattle populations. Signal transducer and activator of transcription 6 (STAT6) gene is tightly connected to IL-4 and IL-13 signalling and plays a key role in T(H)2 polarization of the immune system. In addition, STAT6 acts as a mediator of leptin signalling and has been associated with body weight regulation. The objective of this study was to determine if SNPs within the bovine STAT6 gene are associated with economically important traits in feedlot cattle. The approach consisted of resequencing STAT6 using a panel of DNA from unrelated animals of different beef breeds. Specifically, 16 kb of STAT6 was resequenced in 47 animals and the process revealed 39 SNPs. From the 39 SNPs, a panel of 15 tag SNPs was genotyped in 1500 beef cattle samples with phenotypes to perform a marker-trait association analysis. Among the 15 tag SNPs, five and six were polymorphic in Bos taurus and Bos indicus respectively. An association analysis was performed between the 15 tag SNPs and 14 performance and production traits. SNP ss115492459:C > A, ss115492461:A > G and ss115492458:G > C were significantly associated with back fat, calculated yield grade, cutability, hot carcass weight, dry matter intake, days on feed, back fat rate and average daily gain. These three SNPs were present in all Bos taurus beef breeds examined. Our results provide evidence that polymorphisms in STAT6 are associated with carcass and growth efficiency traits, and may be used for marker-assisted selection and management in feedlot cattle.
鉴定数量性状的基因和多态性，并了解这些基因和多态性如何影响经济性状，对于商业牛群中成功的标记辅助选择和更有效的管理策略非常重要。信号转导子和转录激活子 6（STAT6）基因与 IL-4 和 IL-13 信号紧密相连，在免疫系统的 TH2 极化中发挥关键作用。此外，STAT6 作为瘦素信号的介质，与体重调节有关。本研究的目的是确定牛 STAT6 基因内的 SNPs 是否与肥育牛的重要经济性状相关。该方法包括使用不同牛肉品种的无亲缘关系动物的 DNA 进行 STAT6 测序。具体来说，在 47 只动物中重新测序了 16kb 的 STAT6，该过程揭示了 39 个 SNPs。在 39 个 SNP 中，选择了 15 个标签 SNP 进行 1500 个牛肉样本的基因型分析，并对表型进行标记-性状关联分析。在 15 个标签 SNP 中，有 5 个和 6 个 SNP 在牛和印度牛中分别多态性。对 15 个标签 SNP 与 14 个性能和生产性状进行关联分析。SNP ss115492459：C > A、ss115492461：A > G 和 ss115492458：G > C 与背膘、计算产肉等级、可切割性、热胴体重、干物质摄入量、饲养天数、背膘率和平均日增重显著相关。这三个 SNP 存在于所有检查的牛属牛肉品种中。我们的结果提供了证据表明 STAT6 中的多态性与胴体和生长效率性状相关，可用于肥育牛的标记辅助选择和管理。
