Cheong Hyun Sub, Yoon Du-Hak, Kim Lyoung Hyo, Park Byung Lae, Choi Yoo Hyun, Chung Eui Ryong, Cho Yong Min, Park Eng Woo, Cheong Il-Cheong, Oh Sung-Jong, Yi Sung-Gon, Park Taesung, Shin Hyoung Doo
Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul, 153-803, Korea.
BMC Genet. 2006 Jun 3;7:35. doi: 10.1186/1471-2156-7-35.
Cold carcass weight (CW) and longissimus muscle area (EMA) are the major quantitative traits in beef cattle. In this study, we found several polymorphisms of growth hormone-releasing hormone (GHRH) gene and examined the association of polymorphisms with carcass traits (CW and EMA) in Korean native cattle (Hanwoo).
By direct DNA sequencing in 24 unrelated Korean cattle, we identified 12 single nucleotide polymorphisms within the 9 kb full gene region, including the 1.5 kb promoter region. Among them, six polymorphic sites were selected for genotyping in our beef cattle (n = 428) and five marker haplotypes (frequency > 0.1) were identified. Statistical analysis revealed that -4241A>T showed significant associations with CW and EMA.
Our findings suggest that polymorphisms in GHRH might be one of the important genetic factors that influence carcass yield in beef cattle. Sequence variation/haplotype information identified in this study would provide valuable information for the production of a commercial line of beef cattle.
冷胴体重（CW）和背最长肌面积（EMA）是肉牛的主要数量性状。在本研究中，我们发现了生长激素释放激素（GHRH）基因的几个多态性，并检测了这些多态性与韩牛胴体性状（CW和EMA）的关联。
通过对24头无亲缘关系的韩国牛进行直接DNA测序，我们在9 kb的完整基因区域（包括1.5 kb的启动子区域）内鉴定出12个单核苷酸多态性。其中，选择了6个多态性位点对我们的肉牛（n = 428）进行基因分型，并鉴定出5种标记单倍型（频率> 0.1）。统计分析表明，-4241A>T与CW和EMA显著相关。
我们的研究结果表明，GHRH基因的多态性可能是影响肉牛胴体产量的重要遗传因素之一。本研究中鉴定的序列变异/单倍型信息将为肉牛商业品系的生产提供有价值的信息。
