Zhang Wei, Yeo Mei Ching, Tang Fang Yin, Popovich David G
Department of Chemistry, National University of Singapore, Singapore.
Food Chem Toxicol. 2009 Sep;47(9):2202-8. doi: 10.1016/j.fct.2009.06.006. Epub 2009 Jun 9.
Soyasaponins are bioactive oleanane triterpenoids found in soy and other legumes. The effect of two methanolic extractions of soy flour, room temperature (RT) and reflux (RE) extractions on composition and bioactive properties in hepatocarcinoma cells (Hep-G2) were investigated. A greater amount of 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) conjugated soyasaponins betag was measured in RT and a greater amount of the structurally related non-DDMP soyasaponins I and III were detected in RE. MTT cell viability yielded an LC50 of 0.926+/-0.08 mg/mL for RT and 0.546+/-0.06 mg/mL for RE. ViaCount viability assay showed similar results for RE as the MTT assay however, RT treatment produced no difference compared with the control. Analysis using TUNEL and cell cycle analysis revealed that RE treatment induced apoptosis and flow cytometry forward side scatter and morphological assessment of RT showed evidence of Hep-G2 differentiation after 72 h. Differences in the bioactivities may be attributed to the different concentration of DDMP conjugated soyasaponin betag recovered in RT and RE extracts.
大豆皂苷是存在于大豆和其他豆类中的具有生物活性的齐墩果烷三萜类化合物。研究了大豆粉的两种甲醇提取物，即室温（RT）提取物和回流（RE）提取物对肝癌细胞（Hep-G2）的成分和生物活性的影响。在室温提取物中检测到的2,3-二氢-2,5-二羟基-6-甲基-4H-吡喃-4-酮（DDMP）共轭大豆皂苷βg的含量更高，而在回流提取物中检测到的结构相关的非DDMP大豆皂苷I和III的含量更高。MTT细胞活力检测显示，室温提取物的半数致死浓度（LC50）为0.926±0.08 mg/mL，回流提取物的LC50为0.546±0.06 mg/mL。ViaCount活力检测显示回流提取物的结果与MTT检测相似，然而，室温提取物处理与对照组相比没有差异。使用TUNEL和细胞周期分析进行的分析表明，回流提取物处理可诱导细胞凋亡，而室温提取物处理72小时后的流式细胞仪前向散射和形态学评估显示有Hep-G2分化的迹象。生物活性的差异可能归因于室温提取物和回流提取物中回收的DDMP共轭大豆皂苷βg浓度不同。
