Renal Division; Department of Medicine; Washington University School of Medicine; St. Louis, Missouri USA.
Organogenesis. 2004 Oct;1(2):52-4. doi: 10.4161/org.1.2.1292.
To characterize actions of vitamin D3 on metanephroi transplanted from rat embryos to adult recipients, we incubated metanephroi with or without 0.01, 0.1 or 1 ug/ml vitamin D3, 25-hydroxyvitamin D(3) [25(OH)D(3)] or 1, 25-hydroxyvitamin D(3) [1,25(OH)2D(3)] prior to implantation. The number of glomeruli in developed metanephroi three weeks post-transplantation that had been incubated with 1.0 ug/ml vitamin D(3) was increased relative to the number in metanephroi that were not incubated with vitamin D(3) (control), an effect that was not recapitulated by administration of vitamin D(3) directly to hosts at the time of transplantation. Incubation of metanephroi with 1.0 ug/ml vitamin D(3) also enhanced inulin clearances of metanephroi measured at 12 weeks post-transplantation. The hydroxylated derivative of vitamin D(3), 25(OH)D(3), increased glomerulus number when applied at 0.01 ug/ml but not at higher concentrations, while the twice-hydroxylated derivative 1,25(OH)(2)D(3), failed to increase glomerulus number at any concentration tested. We conclude that incubation with vitamin D(3) prior to implantation enhances inulin clearance possibly by increasing the number of glomeruli that develop post-transplantation.Our findings suggest the vitamin D(3) effect is mediated locally.
为了研究维生素 D3 对大鼠胚胎移植到成年受者后肾的作用，我们在植入前用 0.01、0.1 或 1ug/ml 维生素 D3、25-羟维生素 D（3）[25（OH）D（3）]或 1,25-羟维生素 D（3）[1,25（OH）2D（3）]孵育肾单位。与未孵育维生素 D3（对照）的肾单位相比，植入前用 1.0ug/ml 维生素 D3 孵育的发育肾单位中形成的肾小球数量增加，而直接在移植时向宿主给予维生素 D3 则不能再现这种作用。用 1.0ug/ml 维生素 D3 孵育肾单位还增强了移植后 12 周时测量的肾单位对菊粉的清除率。维生素 D3 的羟化衍生物 25（OH）D3 在 0.01ug/ml 时增加肾小球数量，但在较高浓度时则没有，而两次羟化的衍生物 1,25（OH）2D3 在任何测试浓度下均未能增加肾小球数量。我们的结论是，植入前用维生素 D3 孵育可增强菊粉清除率，可能是通过增加移植后发育的肾小球数量。我们的发现表明，维生素 D3 的作用是局部介导的。
