口腔癌前病变和恶性病变中细胞内超氧化物歧化酶改变的特征：与淋巴结转移的相关性
Characterization of intracellular superoxide dismutase alterations in premalignant and malignant lesions of the oral cavity: correlation with lymph node metastasis.
作者信息
Yokoe Hidetaka, Nomura Hitomi, Yamano Yukio, Fushimi Kazuaki, Sakamoto Yosuke, Ogawara Katsunori, Shiiba Masashi, Bukawa Hiroki, Uzawa Katsuhiro, Takiguchi Yuichi, Tanzawa Hideki
机构信息
Division of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
出版信息
J Cancer Res Clin Oncol. 2009 Nov;135(11):1625-33. doi: 10.1007/s00432-009-0610-8. Epub 2009 Jun 12.
PURPOSE
The purpose of this study was to characterize changes in the expression of copper-zinc superoxide dismutase (Cu/Zn-SOD) and manganese SOD (Mn-SOD) in oral squamous-cell carcinoma (OSCC).
METHODS
Real-time quantitative reverse transcriptase-polymerase chain reaction analysis of Cu/Zn-SOD and Mn-SOD mRNA expression was carried out in 50 pairs of OSCC tissue specimens and corresponding normal tissues. Mn-SOD protein expression was evaluated further in 65 OSCC tissue samples and 33 oral premalignant lesions (OPLs) using immunohistochemistry.
RESULTS
Significant (P < 0.001) upregulation of Mn-SOD mRNA expression was observed in OSCC tissues compared with the normal tissue counterparts, whereas no significant difference was detected in Cu/Zn-SOD expression. Significant increases in Mn-SOD protein expression were seen in both OPLs (P < 0.001) and OSCC tissue (P < 0.001) together with a high incidence of lymph node metastasis (P = 0.04).
CONCLUSIONS
Our findings suggested that Mn-SOD overexpression is a frequent and early event during oral carcinogenesis and could contribute to aggressive OSCC.
目的
本研究旨在描述口腔鳞状细胞癌（OSCC）中铜锌超氧化物歧化酶（Cu/Zn-SOD）和锰超氧化物歧化酶（Mn-SOD）表达的变化。
方法
对50对OSCC组织标本及相应正常组织进行Cu/Zn-SOD和Mn-SOD mRNA表达的实时定量逆转录-聚合酶链反应分析。使用免疫组织化学对65例OSCC组织样本和33例口腔癌前病变（OPL）进一步评估Mn-SOD蛋白表达。
结果
与正常组织相比，OSCC组织中Mn-SOD mRNA表达显著上调（P < 0.001），而Cu/Zn-SOD表达未检测到显著差异。在OPL（P < 0.001）和OSCC组织（P < 0.001）中均观察到Mn-SOD蛋白表达显著增加，且淋巴结转移发生率较高（P = 0.04）。
结论
我们的研究结果表明，Mn-SOD过表达是口腔癌发生过程中常见的早期事件，可能导致侵袭性OSCC。
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