Ohashi Rina, Tajima Ken, Takahashi Fumiyuki, Cui Ri, Gu Tao, Shimizu Kazue, Nishio Kazuto, Fukuoka Kazuya, Nakano Takashi, Takahashi Kazuhisa
Department of Respiratory Medicine, Juntendo University, School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan.
Anticancer Res. 2009 Jun;29(6):2205-14.
Although serum osteopontin (OPN) concentration is elevated in patients with malignant pleural mesothelioma (MPM), the role of OPN in the pathogenesis and development of MPM remains unknown.
To determine the roles of OPN in MPM, immunohistochemical staining was performed to investigate the concentration of OPN in the pleural tumor of patients with mesothelioma; cell adhesion, proliferation and migration assays of H28 cells, an MPM cell line, were also carried out in vitro.
H28 cells cultured on OPN-coated plates revealed enhanced adhesion, proliferation, migration, cell survival and phosphorylated focal adhesion kinase activities. As expected, these enhancements were markedly suppressed with the addition of anti-alphavbeta3 antibody or arginine-glycine-aspartic acid serine (RGDS) peptide to the medium.
OPN is speculated to play an important role in the enhancement of adhesion, proliferation and migration activities of H28 cells, presumably by interacting with the alphavbeta3 integrin.
尽管恶性胸膜间皮瘤（MPM）患者血清骨桥蛋白（OPN）浓度升高，但OPN在MPM发病机制和发展中的作用仍不清楚。
为确定OPN在MPM中的作用，进行免疫组织化学染色以研究间皮瘤患者胸膜肿瘤中OPN的浓度；还在体外对MPM细胞系H28细胞进行了细胞黏附、增殖和迁移试验。
在涂有OPN的平板上培养的H28细胞显示出增强的黏附、增殖、迁移、细胞存活和磷酸化黏着斑激酶活性。正如预期的那样，向培养基中添加抗αvβ3抗体或精氨酸-甘氨酸-天冬氨酸-丝氨酸（RGDS）肽可显著抑制这些增强作用。
推测OPN可能通过与αvβ3整合素相互作用，在增强H28细胞的黏附、增殖和迁移活性中发挥重要作用。
