不同灵长类动物中胸苷激酶基因与半乳糖激酶基因之间的连锁关系。
Linkage relationship between the genes for thymidine kinase and galactokinase in different primates.
作者信息
Orkwiszewski K G, Tedesco T A, Mellman W J, Croce C M
出版信息
Somatic Cell Genet. 1976 Jan;2(1):21-6. doi: 10.1007/BF01539239.
In this study we investigated the expression of primate galactokinase in somatic cell hybrids between a thymidine kinase-deficient mouse cell line and two different primate cell lines, one of which was derived from African green monkey kidney cells and the other from chimpanzee fibroblasts. All the African green monkey-mouse hybrid clones, selected in HAT medium, expressed monkey galactokinase activity and contained a monkey chromosome similar to a human E-group chromosome. When these clones were backselected in medium containing 5-bromodeoxyuridine, both this chromosome and the monkey galactokinase activity were lost. All the hybrid clones between mouse and chimpanzee cells, which were selected in HAT medium, contained the chimpanzee chromosome 17 and expressed chimpanzee galactokinase activity. These results indicate that the linkage relationship between galactokinase and thymidine kinase has been maintained in 3 divergent primate species--man, chimpanzee, and Old World monkey.
在本研究中，我们调查了灵长类半乳糖激酶在胸苷激酶缺陷型小鼠细胞系与两种不同灵长类细胞系（其中一种源自非洲绿猴肾细胞，另一种源自黑猩猩成纤维细胞）之间的体细胞杂种中的表达情况。在HAT培养基中筛选出的所有非洲绿猴 - 小鼠杂种克隆均表达猴半乳糖激酶活性，并含有一条与人类E组染色体相似的猴染色体。当这些克隆在含有5 - 溴脱氧尿苷的培养基中再次筛选时，这条染色体和猴半乳糖激酶活性均丧失。在HAT培养基中筛选出的所有小鼠与黑猩猩细胞之间的杂种克隆均含有黑猩猩17号染色体，并表达黑猩猩半乳糖激酶活性。这些结果表明，半乳糖激酶与胸苷激酶之间的连锁关系在人类、黑猩猩和旧世界猴这3种不同的灵长类物种中得以维持。
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