通过评估标记基因fucK、hap和sodC在常规临床样本中检测被误鉴定为流感嗜血杆菌的隐匿基因种。
Detection of cryptic genospecies misidentified as Haemophilus influenzae in routine clinical samples by assessment of marker genes fucK, hap, and sodC.
作者信息
Nørskov-Lauritsen Niels
机构信息
Department of Clinical Microbiology, Aarhus University Hospital Skejby, Denmark.
出版信息
J Clin Microbiol. 2009 Aug;47(8):2590-2. doi: 10.1128/JCM.00013-09. Epub 2009 Jun 17.
Abstract
Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as variant strains ("nonhemolytic Haemophilus haemolyticus").
摘要
通过与基因特异性探针杂交，评估流感嗜血杆菌临床分离株中fucK、hap和sodC的存在情况，并通过表型和16S rRNA基因测序对与预期流感嗜血杆菌基因型不同的分离株进行特征分析。480株分离株中有两株最终被归类为变异株（“非溶血性溶血嗜血杆菌”）。
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