使用针对编码16S rRNA的基因的特异性引物，通过聚合酶链反应（PCR）对引起泌尿生殖系统和新生儿感染的嗜血杆菌隐性基因种进行基因鉴定。
Genetic identification of cryptic genospecies of Haemophilus causing urogenital and neonatal infections by PCR using specific primers targeting genes coding for 16S rRNA.
作者信息
Quentin R, Ruimy R, Rosenau A, Musser J M, Christen R
机构信息
Département de Microbiologie Médicale et Moléculaire, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1334, CHU Bretonneau, Tours, France.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1380-5. doi: 10.1128/jcm.34.6.1380-1385.1996.
Abstract
Previous genetic analysis of Haemophilus influenzae strains isolated from genital and neonatal infections identified a group of biotype IV that constitutes a cryptic genospecies only distantly related to H. influenzae and H. Haemolyticus. Small-subunit rRNA genes of two representative strains of this genital Haemophilus genospecies (strains 16N and 2406) were sequenced. The analysis indicated that these strains form a monophyletic unit with H. haemolyticus and H. influenzae biogroups Influenzae and Aegyptius and are more closely related to H. haemolyticus than to H. influenzae biogroups Influenzae and Aegyptius. 16S rRNA gene sequences were used to formulate primers for PCR-based identification of cryptic genital Haemophilus organisms. A 242-bp fragment was amplified from strains belonging to the genital Haemophilus genospecies but not from strains of 12 other Haemophilus species, including strains of H. influenzae biotype IV sensu stricto.
摘要
先前对从生殖器和新生儿感染中分离出的流感嗜血杆菌菌株进行的基因分析，鉴定出一组生物型IV，它们构成了一个隐性基因种，与流感嗜血杆菌和溶血嗜血杆菌仅有远缘关系。对该生殖器嗜血杆菌基因种的两个代表性菌株（16N和2406菌株）的小亚基rRNA基因进行了测序。分析表明，这些菌株与溶血嗜血杆菌以及流感嗜血杆菌生物群流感型和埃及型形成一个单系群，并且与溶血嗜血杆菌的关系比与流感嗜血杆菌生物群流感型和埃及型的关系更密切。16S rRNA基因序列被用于设计引物，以基于PCR鉴定隐性生殖器嗜血杆菌菌株。从属于生殖器嗜血杆菌基因种的菌株中扩增出一个242 bp的片段，但从包括严格意义上的IV型流感嗜血杆菌菌株在内的其他12种嗜血杆菌菌株中未扩增出该片段。
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