Center for AIDS Research and Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
RNA Biol. 2009 Sep-Oct;6(4):384-6. doi: 10.4161/rna.6.4.8920. Epub 2009 Sep 1.
Productive HIV-1 replication is mainly controlled at the transcriptional level by HIV-1 Tat and at the post-transcriptional level by HIV-1 Rev. A number of host factors have been identified to be involved in these processes. Src-associated protein of 68 kDa in mitosis (Sam68) is a multi-functional RNA-binding protein and has been implicated in several important cellular processes. More evidence has accumulated to support an important regulatory function of Sam68 at two distinct steps of HIV-1 gene expression. Sam68 interacts with HIV-1 Rev protein and directly participates in nuclear exportation of HIV-1 unspliced or singly spliced RNA. In addition, Sam68 functions in the cytoplasmic processes of HIV-1 replication such as the translational regulation of HIV-1 RNA. Elucidation of the precise molecular function of Sam68 in HIV-1 gene expression is clearly warranted and is expected to unambiguously establish Sam68 as an important host factor for HIV-1 replication.
HIV-1 的转录水平主要由 HIV-1 Tat 控制，转录后水平主要由 HIV-1 Rev 控制。已经确定了许多宿主因子参与这些过程。有丝分裂中与Src 相关的 68kDa 蛋白（Sam68）是一种多功能 RNA 结合蛋白，参与了几个重要的细胞过程。越来越多的证据表明，Sam68 在 HIV-1 基因表达的两个不同步骤中具有重要的调节功能。Sam68 与 HIV-1 Rev 蛋白相互作用，并直接参与 HIV-1 未剪接或单剪接 RNA 的核输出。此外，Sam68 还参与 HIV-1 复制的细胞质过程，如 HIV-1 RNA 的翻译调控。阐明 Sam68 在 HIV-1 基因表达中的精确分子功能是非常必要的，这有望明确确立 Sam68 作为 HIV-1 复制的重要宿主因子。
