Stoll Heather M, Shimizu Nobumichi
Department of Geology, Universidad de Oviedo, Arias de Velasco Oviedo, Asturias, Spain.
Nat Protoc. 2009;4(7):1038-43. doi: 10.1038/nprot.2009.83.
This protocol describes a technique for picking individual nannofossils for geochemical analysis. The protocol allows for preparation of both individual nannofossil specimens and monospecific populations for analysis. A micromanipulator is adapted on an inverted microscope to hold a very fine tungsten needle capable of picking up individual selected nannofossils from an uncovered glass slide. Nannofossils can then be placed in designated areas on a Tacky Dot slide. Subsequently, they can be transferred to epoxy resin and disinterred from the Tacky slide. Nannofossils prepared using this protocol are suitable for subsequent analysis by secondary ion mass spectrometry. The preparation procedure in total takes 90-120 min per sample.
本方案描述了一种挑选单个超微化石用于地球化学分析的技术。该方案允许制备单个超微化石标本和单种群体进行分析。在倒置显微镜上适配一个显微操作器，以固定一根非常细的钨针，该钨针能够从未覆盖的载玻片上拾取单个选定的超微化石。然后可以将超微化石放置在粘性点载玻片上的指定区域。随后，它们可以转移到环氧树脂中，并从粘性载玻片上挖出。使用本方案制备的超微化石适用于随后的二次离子质谱分析。每个样品的制备过程总共需要90 - 120分钟。
