Institut für Spezielle Zoologie und Evolutionsbiologie mit Phyletischem Museum, Friedrich-Schiller-Universität Jena, Jena, Germany.
Microsc Res Tech. 2013 Mar;76(3):258-62. doi: 10.1002/jemt.22161. Epub 2013 Jan 7.
We describe a simple, effective and relatively cheap instrument for the inflation of soft parts of insect genitalia, particularly the vesica (endophallus) of small male lepidopteran specimens. It can also be applied in female genitalia and for the hyperextension of postabdominal segments of the telescoping type. A fine glass capillary is mounted on a self-constructed micromanipulator system with capillary holder, moveably placed aside a microscope. The micromanipulator is constructed from the x- and y-pinions of a discarded microscope table and the z-pinion of a discarded microscope. Preparation is carried out as usual on a microscopic slide. The inflatable soft parts are pushed out mechanically and then fully inflated and subsequently hardened by pumping absolute alcohol with a medical syringe via a flexible PVC tube, the capillary holder and the glass capillary into the phallus. With affordable effort, our "hands-off"apparatus might considerably ease preparation.
我们描述了一种简单、有效且相对廉价的仪器，用于充气昆虫生殖器的柔软部分，特别是小型鳞翅目雄性标本的 vesica（内阴）。它也可应用于雌性生殖器，并可用于伸缩式后腹部节段的过度伸展。一根细玻璃毛细管安装在自制的微操作器系统上，带有毛细管支架，可移动地放置在显微镜旁边。微操作器由废弃显微镜台的 x 和 y 翅片以及废弃显微镜的 z 翅片构建而成。准备工作通常在显微镜载玻片上进行。通过医用注射器通过柔性 PVC 管、毛细管支架和玻璃毛细管将绝对酒精泵入阴茎，将可充气的柔软部分机械推出并完全充气，然后硬化。使用负担得起的努力，我们的“非接触式”仪器可能会大大简化准备工作。
