Bonar R A, Reich C F, Sharief Y
Urol Res. 1977;5(2):87-94. doi: 10.1007/BF00256846.
In a qualitative and quantitative study of enzymic dispersion of cells from the mucosal layer stripped from canine urinary bladder, trypsin was found to be equal or superior to the other enzymes tested for dispersal of urothelial cells specifically. Collagenase or collagenase plus trypsin served to disperse the whole tissue. A procedure for recovering the urothelial cells as a single-cell suspension and establishing them in culture is presented. The morphology, culture behaviour, and chromosome complement of these cells is described.
在一项对从犬膀胱黏膜层剥离的细胞进行酶分散的定性和定量研究中，发现胰蛋白酶在特异性分散尿路上皮细胞方面与其他测试酶相当或更优。胶原酶或胶原酶加胰蛋白酶用于分散整个组织。本文介绍了一种将尿路上皮细胞作为单细胞悬液回收并进行培养的方法。描述了这些细胞的形态、培养行为和染色体组成。
