Sassani A, Mirchamsy H, Shafyi A, Ahourai P, Razavi J, Gholami M R, Mohammadi A, Ezzi A, Rahmani M, Fateh G
Razi State Serum and Vaccine Institute, Tehran, Iran.
Biologicals. 1991 Jul;19(3):203-11. doi: 10.1016/1045-1056(91)90036-j.
A new live attenuated mumps vaccine was developed in human diploid cells. The S-12 virus was isolated from a 10-year-old girl showing typical symptoms of mumps infection, the diagnosis was confirmed by a pediatrician. The virus was isolated in green monkey kidney cells, without passage in chick embryo cavity or chick embryo fibroblasts. Attenuation of the wild virus was performed by serial passages in human diploid cells (MRC-5). The attenuated virus was characterized by identity tests, as well as by a reduction in plaque size, as marker tests. The virus was free from adventitious agents and safe for laboratory animals as well as for monkeys. The reactogenicity and immunogenicity of the S-12 virus for man was investigated by administration of a monovalent vaccine to 20 seronegative adult male volunteers and 30 children aged 1 to 5 years without history of mumps infection or vaccination. Seroconversion was obtained in 95% of the vaccinees. The new vaccine has the advantage of not requiring specific pathogen-free eggs, and being free from avian proteins and therefore can be used in sensitized patients.
一种新的减毒活腮腺炎疫苗是在人二倍体细胞中研制出来的。S - 12病毒是从一名出现腮腺炎感染典型症状的10岁女孩身上分离出来的，诊断由一名儿科医生确认。该病毒在绿猴肾细胞中分离，未在鸡胚腔或鸡胚成纤维细胞中传代。野生病毒的减毒是通过在人二倍体细胞（MRC - 5）中连续传代进行的。减毒病毒通过同一性试验以及噬斑大小减小作为标记试验进行鉴定。该病毒无外源因子，对实验动物和猴子均安全。通过给20名血清阴性成年男性志愿者和30名1至5岁无腮腺炎感染或疫苗接种史的儿童接种单价疫苗，研究了S - 12病毒对人的反应原性和免疫原性。95%的接种者出现了血清转化。这种新疫苗的优点是不需要特定的无特定病原体鸡蛋，且不含禽蛋白，因此可用于过敏患者。
