化学发光底物可提高蛋白质免疫印迹中抗原检测的灵敏度。
Chemiluminescent substrates increase sensitivity of antigen detection in western blots.
作者信息
Sandhu G S, Eckloff B W, Kline B C
机构信息
Dept. of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, MN 55905.
出版信息
Biotechniques. 1991 Jul;11(1):14-6.
PMID:1954010
Abstract
Replacement of colorimetric substrates in Western blots by chemiluminescent reagents enhances the sensitivity of detection by more than an order of magnitude. Protein levels beyond the detection limit of colorimetric substrates can be consistently detected without modifying pre-existing laboratory protocols.
摘要
在蛋白质免疫印迹中，用化学发光试剂替代比色底物可将检测灵敏度提高一个数量级以上。无需修改现有的实验室方案，就能始终检测出超出比色底物检测限的蛋白质水平。
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