Dou S, Zeng X, Cortes P, Erdjument-Bromage H, Tempst P, Honjo T, Vales L D
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
Mol Cell Biol. 1994 May;14(5):3310-9. doi: 10.1128/mcb.14.5.3310-3319.1994.
We have identified a cellular protein, RBP-2N, a presumed recombinase, as a repressor of transcription. Inhibition of transcription by RBP-2N was dependent on its DNA recognition site and was demonstrated in vitro and in vivo. This repression appears to be general, as transcription mediated by SP1 and Gal4/VP16 was inhibited by RBP-2N. The protein was purified to near homogeneity from human cells on the basis of its binding to a site present in the promoter of the adenovirus pIX gene. The DNA recognition sequence is 5'-TGGGAAAGAA, which is markedly different from the recombination signal sequence originally identified as the target site for this protein. The sequence of the purified protein is 97% identical with that published for the mouse RBP-2N protein. The reported homolog in Drosophila is Suppressor of Hairless. RBP-2N binding sites are present in a number of cellular and viral promoters, so RBP-2N may have a general role in transcriptional repression.
我们已鉴定出一种细胞蛋白RBP - 2N，一种推测的重组酶，作为转录抑制因子。RBP - 2N对转录的抑制作用依赖于其DNA识别位点，并在体外和体内得到证实。这种抑制作用似乎具有普遍性，因为由SP1和Gal4/VP16介导的转录也受到RBP - 2N的抑制。基于该蛋白与腺病毒pIX基因启动子中一个位点的结合，从人细胞中纯化得到了近乎均一的该蛋白。其DNA识别序列为5'-TGGGAAAGAA，这与最初鉴定为该蛋白靶位点的重组信号序列明显不同。纯化蛋白的序列与已发表的小鼠RBP - 2N蛋白序列有97%的同一性。在果蝇中报道的同源物是无毛抑制因子。RBP - 2N结合位点存在于许多细胞和病毒启动子中，因此RBP - 2N可能在转录抑制中具有普遍作用。
