Wang Fang, Guo Xin, Ge Xinna, Wang Zhongtian, Chen Yanhong, Cha Zhenlin, Yang Hanchun
Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Virus Res. 2009 Oct;145(1):151-6. doi: 10.1016/j.virusres.2009.05.015. Epub 2009 Jun 18.
Forty Chinese PCV2 strains collected between 2004 and 2008 were sequenced and their genetic variations were analyzed together with nine previous PCV2 isolates. Phylogenetic analysis indicated that these Chinese PCV2 strains could be divided into four genotypes (PCV-2a, PCV-2b, PCV-2d and PCV-2e), and the genotype PCV-2c defined in Denmark was not found. PCV-2d and PCV-2e were two genotypes firstly determined in our study. Variation analysis of amino acids of capsid protein revealed that Chinese PCV2 strains clustered within PCV-2d had four amino acid marker positions and the isolates within PCV-2e had seven unique amino acid mutations. Our analysis also showed that PCV-2b became dominating in China in recent years. These data contribute to the understanding of PCV2 molecular epidemiology.
对2004年至2008年间收集的40株中国猪圆环病毒2型（PCV2）毒株进行测序，并与之前的9株PCV2分离株一起分析其基因变异情况。系统发育分析表明，这些中国PCV2毒株可分为4种基因型（PCV-2a、PCV-2b、PCV-2d和PCV-2e），未发现丹麦定义的PCV-2c基因型。PCV-2d和PCV-2e是本研究首次确定的两种基因型。衣壳蛋白氨基酸变异分析显示，PCV-2d基因型内的中国PCV2毒株有4个氨基酸标记位点，PCV-2e基因型内的分离株有7个独特的氨基酸突变。我们的分析还表明，近年来PCV-2b在中国占主导地位。这些数据有助于了解PCV2的分子流行病学。
