University of Veterinary Medicine and Pharmacy, Department of Epizootiology and Parasitology, Komenskeho 73, 041 81 Kosice, Slovakia.
Res Vet Sci. 2011 Feb;90(1):168-73. doi: 10.1016/j.rvsc.2010.04.022. Epub 2010 May 23.
Of 120 clinical specimens obtained from pigs bred on 28 PMWS-affected farms in Slovakia, porcine circovirus type 2 (PCV-2) was detected by single PCR in 77 samples. A short 224 bp fragment of ORF2 was used for preliminary grouping of isolates by phylogenetic analysis. Nucleotide sequences of the entire ORF2 region provided more precise genetic typing and segregation of preselected isolates (n=10) into two known genotypes, PCV-2a (n=1) and PCV-2b (n=9). Complete genome sequencing of three selected isolates allowed their definitive grouping into genotype PCV-2b, cluster 1A or genotype PCV-2a, cluster 2D. No correlation between the mutations and the geographic origin of isolates was observed. Results confirmed that many PCV-2 isolates are genetically very stable since similar viruses circulate in Central and Western Europe.
从斯洛伐克 28 个患有 PMWS 的农场饲养的 120 份临床标本中，通过单次 PCR 在 77 份样本中检测到猪圆环病毒 2 型（PCV-2）。使用 ORF2 的短 224 bp 片段通过系统发育分析对分离株进行初步分组。ORF2 区的核苷酸序列提供了更精确的遗传分型，并将预选分离株（n=10）分为两种已知基因型，PCV-2a（n=1）和 PCV-2b（n=9）。对三个选定分离株的全基因组测序允许它们明确分组为基因型 PCV-2b、簇 1A 或基因型 PCV-2a、簇 2D。未观察到突变与分离株地理起源之间存在相关性。结果证实，由于相似的病毒在中欧和西欧传播，许多 PCV-2 分离株在遗传上非常稳定。
