Lopes K R F, Costa L L M, Lima G L, Souza A L P, Silva A R
Departamento de Ciências Animais, Universidade Federal Rural do Semi-Arido - UFERSA, Costa e Silva, 59625-900, Mossoró, RN, Brazil.
Theriogenology. 2009 Sep 15;72(5):650-4. doi: 10.1016/j.theriogenology.2009.04.021. Epub 2009 Jun 21.
The objective of this study was to compare the effects of dimethylformamide (DMF) and glycerol in canine (Canis lupus familiaris) semen cryopreservation based on postthaw motility and velocity evaluated by computer-assisted sperm analysis (CASA) and the effects on subjective progressive motility, percentage of live sperm, and plasma membrane functional integrity. The semen was diluted in two steps with an egg-yolk Tris extender containing 6% glycerol or DMF, frozen in 0.25-mL straws, and stored in liquid nitrogen. Immediately after thawing, samples were accessed for subjective sperm motility, sperm membrane functional integrity, percentage of live sperm, and evaluation by CASA. There were differences (P<0.05) between glycerol and DMF with regard to subjective progressive motility (43.1% vs. 21.5%), objective progressive motility (11.8% vs. 6.2%), velocity average pathway (31.1 vs. 23.1 microm/sec), and amplitude of lateral head (3.3 vs. 3.9 microm), which confirmed the efficiency of glycerol. In conclusion, objective analysis performed by CASA confirmed that no benefits were derived by using DMF to replace glycerol for cryopreservation of canine semen.
本研究的目的是基于计算机辅助精子分析（CASA）评估的解冻后活力和速度，比较二甲基甲酰胺（DMF）和甘油在犬（家犬）精液冷冻保存中的效果，以及它们对主观渐进性活力、活精子百分比和质膜功能完整性的影响。精液用含有6%甘油或DMF的蛋黄Tris稀释液分两步稀释，装入0.25 mL细管中冷冻，并储存在液氮中。解冻后立即对样品进行主观精子活力、精子膜功能完整性、活精子百分比评估以及CASA评估。甘油和DMF在主观渐进性活力（43.1%对21.5%）、客观渐进性活力（11.8%对6.2%）、平均路径速度（31.1对23.1微米/秒）和头部侧向幅度（3.3对3.9微米）方面存在差异（P<0.05），这证实了甘油的有效性。总之，CASA进行的客观分析证实，使用DMF替代甘油冷冻保存犬精液没有益处。
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