Nazeri E, Niasari-Naslaji A, Ghasemzadeh-Nava H, Panahi F
Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Iran J Vet Res. 2023;24(1):6-13. doi: 10.22099/IJVR.2022.43403.6339.
Plasma egg yolk (PEY), due to simple preparation and easier access, could be a suitable alternative to raw egg yolk for preserving canine semen.
The present study investigated suitable concentrations of PEY and glycerol for preservation of canine semen.
Semen was collected by digital manipulation (seven replicates from four dogs). Following initial raw semen evaluation, the semen was diluted in a tris-based extender supplemented with varying concentrations of chicken PEY (0, 20, and 40% v/v) and glycerol (3%; v/v). After cooling the specimen to 4°C within 1 h, the specimens were diluted with an equal volume of freezing extender consisting of similar concentrations of chicken PEY and 0 and 7% glycerol to reach the final glycerol concentration of 1.5 and 5% for short-term storage of canine semen. Samples with different concentrations of PEY and 5% glycerol were frozen. The sperm viability parameters including total motility, progressive forward motility, plasma membrane integrity, and live percentage of sperm were assessed following short and long-term storage.
Sperm viability parameters of semen extended in an extender supplemented with 20 or 40% chicken PEY with either 1.5 or 5% glycerol remained superior until 72 h after semen collection compared to the specimen that did not receive any PEY (P<0.05). Post-thaw sperm viability was also greater in samples extended in extender supplemented with either 20 or 40% PEY compared to 0% PEY.
Tris-based extender supplemented with either 20% chicken PEY could be suitable for short and long-term preservation of canine semen.
由于制备简单且获取容易，血浆蛋黄（PEY）可能是用于保存犬精液的生蛋黄的合适替代品。
本研究调查了用于保存犬精液的PEY和甘油的合适浓度。
通过指检采集精液（来自4只犬，共7次重复）。在对初始的新鲜精液进行评估后，将精液稀释于添加了不同浓度鸡血浆蛋黄（0、20和40% v/v）和甘油（3%；v/v）的基于Tris的稀释液中。在1小时内将样本冷却至4°C后，用等体积的由相似浓度的鸡血浆蛋黄和0及7%甘油组成的冷冻稀释液进行稀释，以使犬精液短期保存时最终甘油浓度达到1.5%和5%。对具有不同浓度PEY和5%甘油的样本进行冷冻。在短期和长期保存后评估精子活力参数，包括总活力、直线前向运动、质膜完整性和精子活率。
与未添加任何PEY的样本相比，添加20%或40%鸡血浆蛋黄以及1.5%或5%甘油的稀释液中保存的精液，其精子活力参数在采精后72小时内仍保持较高水平（P<0.05）。与0% PEY相比，添加20%或40% PEY的稀释液中保存的样本解冻后的精子活力也更高。
添加20%鸡血浆蛋黄的基于Tris的稀释液可能适用于犬精液的短期和长期保存。
