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白色沉积物芽孢杆菌新种,一种从高盐湖中分离出的中度嗜盐革兰氏阳性细菌,以及对沉积物芽孢杆菌属(Carrasco等人,2008年)的修订描述。

Sediminibacillus albus sp. nov., a moderately halophilic, Gram-positive bacterium isolated from a hypersaline lake, and emended description of the genus Sediminibacillus Carrasco et al. 2008.

作者信息

Wang Xiaowei, Xue Yanfen, Ma Yanhe

机构信息

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, PR China.

出版信息

Int J Syst Evol Microbiol. 2009 Jul;59(Pt 7):1640-4. doi: 10.1099/ijs.0.004234-0. Epub 2009 Jun 19.

Abstract

A moderately halophilic, Gram-positive-staining, endospore-forming, rod-shaped and strictly aerobic bacterium, designated strain NHBX5(T), was isolated from Lake Nanhuobuxun in China. Strain NHBX5(T) was able to grow at NaCl concentrations of 0-22 % (w/v) (optimally at 7 %, w/v), at pH 6.0-9.0 (optimally at pH 7.5) and at temperatures of 10-45 degrees C (optimally at 37 degrees C). The cell-wall peptidoglycan of strain NHBX5(T) contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and a glycolipid. The genomic DNA G+C content of strain NHBX5(T) was 44.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NHBX5(T) was most closely related to Sediminibacillus halophilus EN8d(T) (98.6 % gene sequence similarity). The level of DNA-DNA relatedness between strain NHBX5(T) and S. halophilus CGMCC 1.6199(T) was 34.6 %. Based on the data presented, strain NHBX5(T) represents a novel species of the genus Sediminibacillus, for which the name Sediminibacillus albus sp. nov. is proposed. The type strain is NHBX5(T) (=DSM 19340(T) =CGMCC 1.6502(T)). In addition, an emended description of the genus Sediminibacillus is presented.

摘要

从中国南湖布尔逊湖分离出一株中度嗜盐、革兰氏阳性、产芽孢、杆状且严格需氧的细菌,命名为菌株NHBX5(T)。菌株NHBX5(T)能够在0-22%(w/v)的NaCl浓度下生长(最适浓度为7%,w/v),在pH 6.0-9.0(最适pH为7.5)以及10-45℃的温度下生长(最适温度为37℃)。菌株NHBX5(T)的细胞壁肽聚糖含有内消旋二氨基庚二酸作为诊断性二氨基酸。主要的类异戊二烯醌为MK-7。主要的细胞脂肪酸为anteiso-C(15 : 0)和anteiso-C(17 : 0)。极性脂为磷脂酰甘油、双磷脂酰甘油和一种糖脂。菌株NHBX5(T)的基因组DNA G+C含量为44.9 mol%。基于16S rRNA基因序列比较的系统发育分析表明,菌株NHBX5(T)与嗜盐沉积物芽孢杆菌EN8d(T)关系最为密切(基因序列相似性为98.6%)。菌株NHBX5(T)与嗜盐沉积物芽孢杆菌CGMCC 1.6199(T)之间的DNA-DNA相关性水平为34.6%。根据所提供的数据,菌株NHBX5(T)代表沉积物芽孢杆菌属的一个新物种,为此提出名为白色沉积物芽孢杆菌(Sediminibacillus albus sp. nov.)的名称。模式菌株为NHBX5(T)(=DSM 19340(T)=CGMCC 1.6502(T))。此外,还给出了沉积物芽孢杆菌属的修订描述。

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