Chen Yi-Guang, Cui Xiao-Long, Fritze Dagmar, Chai Li-Hong, Schumann Peter, Wen Meng-Liang, Wang Yong-Xia, Xu Li-Hua, Jiang Cheng-Lin
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):647-53. doi: 10.1099/ijs.0.65365-0.
A Gram-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, oxidase- and catalase-positive, rod-shaped bacterium, strain YIM kkny16(T), was isolated from a saline mud sample collected from the Keke salt lake in the Qaidam Basin, north-west China. This isolate grew in the presence of 0-25 % (w/v) NaCl and at pH 6.0-10.0 and 10-50 degrees C; optimum growth was observed with 10 % (w/v) NaCl and at pH 7.0 and 37 degrees C. Strain YIM kkny16(T) had meso-diaminopimelic acid as the diagnostic diamino acid, MK-7 as the predominant respiratory quinone, with a significant amount of MK-6, and anteiso-C(15 : 0), iso-C(14 : 0) and C(16 : 1)omega7c alcohol as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 41.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain YIM kkny16(T) was a member of the genus Virgibacillus, exhibiting sequence similarities of 94.9-97.3 % to the type strains of recognized Virgibacillus species. Strain YIM kkny16(T) could be differentiated from recognized Virgibacillus species based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of evidence from this polyphasic study, strain YIM kkny16(T) is considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus kekensis sp. nov. is proposed. The type strain is YIM kkny16(T) (=DSM 17056(T)=CGMCC 1.6298(T)).
从中国西北部柴达木盆地可可盐湖采集的盐泥样品中分离出一株革兰氏阳性、中度嗜盐、具运动性、严格需氧、产芽孢、氧化酶和过氧化氢酶阳性的杆状细菌，菌株YIM kkny16(T)。该分离株在0-25%（w/v）NaCl、pH 6.0-10.0和10-50℃条件下生长；在10%（w/v）NaCl、pH 7.0和37℃条件下观察到最佳生长。菌株YIM kkny16(T)以中-二氨基庚二酸作为诊断性二氨基酸，以MK-7作为主要呼吸醌，同时含有大量MK-6，以anteiso-C(15 : 0)、iso-C(14 : 0)和C(16 : 1)ω7c醇作为主要脂肪酸。主要极性脂为双磷脂酰甘油和磷脂酰甘油。DNA G+C含量为41.8 mol%。基于16S rRNA基因序列的系统发育分析证实，菌株YIM kkny16(T)是 Virgibacillus 属的成员，与已确认的 Virgibacillus 属模式菌株的序列相似性为94.9-97.3%。基于表型特征、化学分类学差异、系统发育分析和DNA-DNA杂交数据，菌株YIM kkny16(T)可与已确认的 Virgibacillus 属物种区分开来。基于这一多相研究的证据，菌株YIM kkny16(T)被认为代表 Virgibacillus 属的一个新物种，为此提出 Virgibacillus kekensis sp. nov. 这一名称。模式菌株为YIM kkny16(T)（=DSM 17056(T)=CGMCC 1.6298(T)）。
