Carrasco I J, Márquez M C, Xue Y, Ma Y, Cowan D A, Jones B E, Grant W D, Ventosa A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Sevilla, Spain.
Int J Syst Evol Microbiol. 2008 Aug;58(Pt 8):1961-7. doi: 10.1099/ijs.0.65790-0.
A Gram-positive, moderately halophilic bacterium, designated strain EN8d(T), was isolated from sediment from Lake Erliannor in the Inner Mongolia Autonomous Region, China. Cells were facultatively anaerobic, rod-shaped and motile and did not display endospore formation. Isolate EN8dT grew in a complex medium supplemented with 0-20 % (w/v) marine salts (optimally at 5-7.5 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain EN8dT was a member of the family Bacillaceae, belonging to a cluster with Thalassobacillus (96.3 % gene sequence similarity) and Halobacillus (95.0-96.0 %), albeit emerging as an independent lineage from members of these two genera. Strain EN8dT contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed MK-7 as the major respiratory isoprenoid quinone. The major fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The DNA G+C content was 47.5 mol%. Strain EN8dT could be clearly differentiated from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore strain EN8dT is considered to represent a novel genus and species, for which the name Sediminibacillus halophilus gen. nov., sp. nov. is proposed. The type strain of Sediminibacillus halophilus is EN8dT (=CCM 7364T =CECT 7148T =CGMCC 1.6199T =DSM 18088T).
从中国内蒙古自治区二连诺尔湖的沉积物中分离出一株革兰氏阳性中度嗜盐细菌，命名为EN8d(T)。细胞为兼性厌氧，杆状且具运动性，不形成芽孢。分离株EN8dT在添加0 - 20%（w/v）海盐的复合培养基中生长（最适为5 - 7.5%，w/v）。基于16S rRNA基因序列比较的系统发育分析表明，菌株EN8dT是芽孢杆菌科的成员，与嗜盐芽孢杆菌属（基因序列相似性96.3%）和嗜盐芽孢杆菌属（95.0 - 96.0%）属于一个聚类群，尽管它是从这两个属的成员中独立出来的一个谱系。菌株EN8dT含有基于内消旋二氨基庚二酸的细胞壁肽聚糖，以MK - 7作为主要的呼吸类异戊二烯醌。主要脂肪酸为anteiso - C15:0和anteiso - C17:0。极性脂质模式由双磷脂酰甘油、磷脂酰甘油和一种未鉴定的糖脂组成。DNA G + C含量为47.5 mol%。菌株EN8dT可根据一些表型、基因型和化学分类特征与其系统发育近缘种明显区分开来。因此，菌株EN8dT被认为代表一个新的属和种，为此提出新属名嗜盐沉积物芽孢杆菌属（Sediminibacillus halophilus），新种名嗜盐沉积物芽孢杆菌（Sediminibacillus halophilus）。嗜盐沉积物芽孢杆菌的模式菌株为EN8dT（=CCM 7364T =CECT 7148T =CGMCC 1.6199T =DSM 18088T）。
