Díaz Prado Silvia Maria, Medina Villaamil Vanessa, Aparicio Gallego Guadalupe, Blanco Calvo Moisés, López Cedrún José Luis, Sironvalle Soliva Sheila, Valladares Ayerbes Manuel, García Campelo Rosario, Antón Aparicio Luis M
Medicine Department, University of La Coruña, La Coruña, Spain.
Virchows Arch. 2009 Jul;455(1):67-75. doi: 10.1007/s00428-009-0793-z. Epub 2009 Jun 19.
Genes of the Wnt and Frizzled class, expressed in HNSCC tissue and cell lines, have an established role in cell morphogenesis and differentiation, and also they have oncogenic properties. We studied Wnt and Fz genes as potential tumor-associated markers in HNSCC by qPCR. Expression levels of Wnt and Fz genes in 22 unique frozen samples from HNSCC were measured. We also assessed possible correlation between the expression levels obtained in cancer samples in relation to clinicopathologic outcome. Wnt-1 was not expressed in the majority of the HNSCC studied, whereas Wnt-5A was the most strongly expressed by the malignant tumors. Wnt-10B expression levels were related with higher grade of undifferentiation. Related to Fz genes, Fz-5 showed more expression levels in no-affectation of regional lymph nodes. Kaplan-Meier survival analyses suggest a reduced time of survival for low and high expression of Wnt-7A and Fz-5 mRNA, respectively. qPCR demonstrated that HNSCC express Wnt and Fz members, and suggested that Wnt and Fz signaling is activated in HNSCC cells.
Wnt和卷曲蛋白（Frizzled）家族基因在头颈部鳞状细胞癌（HNSCC）组织及细胞系中表达，在细胞形态发生和分化中发挥既定作用，且具有致癌特性。我们通过定量聚合酶链反应（qPCR）研究了Wnt和Fz基因作为HNSCC潜在肿瘤相关标志物的情况。测定了来自HNSCC的22个独特冷冻样本中Wnt和Fz基因的表达水平。我们还评估了癌症样本中获得的表达水平与临床病理结果之间可能存在的相关性。在所研究的大多数HNSCC中，Wnt-1未表达，而Wnt-5A在恶性肿瘤中表达最强。Wnt-10B的表达水平与更高程度的未分化相关。关于Fz基因，Fz-5在无区域淋巴结受累时显示出更高的表达水平。Kaplan-Meier生存分析表明，Wnt-7A和Fz-5 mRNA低表达和高表达时分别提示生存时间缩短。qPCR证明HNSCC表达Wnt和Fz家族成员，并提示Wnt和Fz信号在HNSCC细胞中被激活。
