用于定量血浆蛋白质组分析的电喷雾质谱法。
Electrospray mass spectrometry for quantitative plasma proteome analysis.
作者信息
Wang Hong, Hanash Sam
机构信息
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
出版信息
Methods Mol Biol. 2009;564:227-42. doi: 10.1007/978-1-60761-157-8_13.
Abstract
Electrospray ionization mass spectrometry (ESI-MS) is an efficient soft ionization procedure for macro biomolecules. However, it is a rather delicate process to produce charged molecules for mass-to-charge ratio (m/z) based measurement. In this chapter, the mechanism of ESI is briefly presented, and the experimental pipeline for quantitative profiling of plasma proteins (prefractionation immunodepletion, protein isotope tagging, 2D-HPLC separation of intact proteins, and LC-MS) is presented as applied by our group in studies of cancer biomarker discovery.
摘要
电喷雾电离质谱法（ESI-MS）是一种用于大分子生物分子的高效软电离方法。然而，产生用于基于质荷比（m/z）测量的带电分子是一个相当精细的过程。在本章中，简要介绍了电喷雾电离的机制，并介绍了我们小组在癌症生物标志物发现研究中应用的血浆蛋白定量分析实验流程（预分级免疫去除、蛋白质同位素标记、完整蛋白质的二维高效液相色谱分离以及液相色谱-质谱联用）。
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